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Bio-Protocol|November 13, 2025
Optimized Protocol for the Collection, Cryopreservation, and In Vitro Cultivation of Human Gut Microbiota for Toxicomicrobiomics ApplicationsPaulina Średnicka, Paulina Emanowicz, Michał WójcickiBio-Protocol|November 13, 2025
Computational Workflow for Genome-Wide DNA Methylation Profiling and Differential Methylation AnalysisPei-Yu Lin, Guan-Jun Lin, Kuan-Lin Chen, et al.Bio-Protocol|January 12, 2026
Generating ER-TRG and CA-ER-TRG Knock-in Mice and Quantitative In Vivo Imaging of ER-phagyMengyuan Zhang, Yu Zhang, Yongjuan Sang, et al.Bio-Protocol|January 12, 2026
Optimized Method for High-Quality Isolation of Single-Nuclei From Mosquito Fat Body for RNA SequencingStephanie Serafim de Carvalho, Colton McNinch, Carolina Barillas-MuryBio-Protocol|January 12, 2026
Simultaneous Non-Invasive Electrocardiogram and Respiration Rate Recordings in Head-Fixed Awake MiceHashmat Ghanizada, Ryszard Stefan Gomolka, Maiken NedergaardBio-Protocol|January 12, 2026
Efficient Fluorescent Labeling of Human Trophoblast Stem Cells via a CRISPR/Cas9-Mediated Knock-In Approach in a Safe Harbor LocusHengshan Zhang, Jie Zhou, Morgan Orsolini, et al.Bio-Protocol|December 26, 2025
High Precision Antibody-Free Microtubule Labeling for Expansion MicroscopyRajdeep Chowdhury, Donatus Krah, Antonios Ntolkeras, et al.Bio-Protocol|December 26, 2025
Chloroplast Movement Imaging Under Different Light Regimes With a Hyperspectral CameraPaweł Hermanowicz, Anna Hebda, Justyna ŁabuzBio-Protocol|December 26, 2025
Manufacturing of Living Building Materials With Calcifying CyanobacteriaPatrick Jung, Jan Friedek, Laura Briegel-Williams, et al.Bio-Protocol|December 26, 2025
Stimulation-Guided AAV Delivery and Longitudinal Assessment of Optogenetic Expression in Rat Motor NervesEmma M Moravec, Jordan J WilliamsPageof 385