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Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
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Amnion-derived cells express intercellular adhesion molecule-1: regulation by cytokines

K W Marvin1, W R Hansen, H C Miller

  • 1Department of Pharmacology and Clinical Pharmacology, University of Auckland, Private Bag 92019, Auckland, New Zealand.

Journal of Molecular Endocrinology
|April 9, 1999
PubMed

Insights

Amnion-derived cells express intercellular adhesion molecule-1 (ICAM-1), regulated by tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta). This finding has implications for preterm labor markers and inflammatory processes.

Area of Science:

  • Immunology
  • Reproductive Biology
  • Cell Biology

Background:

  • Intercellular adhesion molecule-1 (ICAM-1) plays a role in immune responses and cell adhesion.
  • The role of ICAM-1 expression in amnion-derived cells and its regulation by inflammatory cytokines is not well understood.

Purpose of the Study:

  • To investigate ICAM-1 mRNA and protein expression in primary and established amnion-derived cells.
  • To determine the regulatory effects of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) on ICAM-1 expression in these cells.

Main Methods:

  • Quantitative analysis of ICAM-1 mRNA expression using established cell lines (WISH) and primary amnion cells.
  • Treatment with TNF-alpha and IL-1beta to assess cytokine-induced changes in ICAM-1 expression.
  • Measurement of ICAM-1 protein levels following cytokine stimulation.
  • Use of cycloheximide to investigate the role of protein synthesis in ICAM-1 induction.

Main Results:

  • TNF-alpha and IL-1beta significantly increased ICAM-1 mRNA expression in WISH cells within 1-2 hours, peaking at 2 hours.
  • ICAM-1 protein levels also increased significantly in WISH cells within 4 hours of cytokine treatment.
  • Basal ICAM-1 mRNA was undetectable in primary amnion cells, but TNF-alpha induced its expression within 2 hours, peaking at 4-8 hours.
  • Cycloheximide did not inhibit TNF-alpha or IL-1beta-induced ICAM-1 mRNA expression, suggesting a post-transcriptional regulatory mechanism.

Conclusions:

  • Amnion-derived cells express ICAM-1, and this expression is upregulated by pro-inflammatory cytokines TNF-alpha and IL-1beta.
  • These findings suggest that the amnion may contribute to soluble ICAM-1 levels and that ICAM-1 could serve as a marker for preterm labor.
  • The amnion's participation in inflammatory processes of the fetal membranes is highlighted, extending beyond its known secretory functions.

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