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Updated: Aug 9, 2026
Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
Published on: May 12, 2015
[Immunohistochemical detection of cytokine receptors on cryostat tissue sections]
A Kumberová1, A Jirásek, K Koubek
1Ustav hematologie a krevní transfuze, Praha.
Insights
This study investigated cytokine receptor expression in various human tissues using advanced immunohistological techniques. The findings highlight the effectiveness of biotin-streptavidin staining for detecting these crucial receptors in disease contexts.
Area of Science:
- Immunohistochemistry
- Molecular Biology
- Oncology
Context:
- Cytokine receptors play vital roles in cellular communication and immune responses.
- Dysregulation of cytokine receptor expression is implicated in various hematological malignancies and autoimmune diseases.
- Accurate detection of cytokine receptors in tissue is essential for understanding disease pathogenesis and developing targeted therapies.
Purpose:
- To evaluate the efficacy of a high-sensitivity biotin-streptavidin system for immunohistological detection of cytokine receptors on cryostat tissue sections.
- To examine the tissue expression patterns of a comprehensive panel of cytokine receptors (including CD25, CD95, CD116, CD117, CD120a/b, CD121a, CDw123, CD124, CD126, CD127, CDw128, CD130, CD131, CD132, CD134, CD135) in various lymphoid tissues.
- To assess the utility of this method in diagnosing and understanding hematological disorders such as non-Hodgkin's lymphoma, myeloma, Hodgkin's disease, autoimmune hemolytic anemia, and thrombocytopenic purpura.
Summary:
- A panel of monoclonal antibodies targeting specific cytokine receptors was employed.
- Immunohistochemistry utilizing a biotin-streptavidin system was performed on cryostat sections of lymph nodes and spleens from patients with diverse hematological conditions.
- The study successfully demonstrated the expression of numerous cytokine receptors, providing detailed tissue-specific expression profiles.
Impact:
- The developed immunohistological technique is highly sensitive and suitable for detecting cytokine receptors in native tissues.
- This method serves as a valuable supplementary staining procedure for research and clinical diagnostics in hematology and immunology.
- Understanding cytokine receptor expression patterns can lead to improved diagnostic markers and therapeutic strategies for hematological malignancies and autoimmune disorders.
Abstract:
We have studied tissue expression of the cytokine receptors using a high sensitivity biotin-streptavidin system on cryostat sections. We used a panel of monoclonal antibodies from the 6th International Workshop on Human Leukocyte Differentiation Antigens, namely CD25 (IL-2R alpha), CD95 (FAS antigen), CD116 (GM CSFR), CD117 (SCFR), CD120 alpha (TNFR I), CD120b (TNFR II), CD121a (IL-1R I), CDw123 (IL-3R), CD124 (IL-4R), CD126 (IL-6R), CD127 (IL-7R), CDw128 (IL-8R), CD130 (gpl130), CD131 (IL-3R), CD132 (IL-2R gamma), CD134 (OC-40), CD135 (FLT3/FLK2). Examined tissues (lymph nodes and spleens) were obtained from 12 patients with folicular non-Hodgkin's lymphoma, periferal T non-Hodgkin's lymphoma, B lymphoma, myeloma, Hodgkin's disease, two cases of T cell rich B-lymphoma, autoimmune haemolytic anemia and two cases of rudimentary trombocytopenic purpura. Our results indicate that immunohistological technology using native tissues on cryostat sections, monoclonal antibodies and the visualisation with biotin-streptavidin is a particularly suitable supplementary staining procedure for detection of the cytokine receptors in tissues.
