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Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes
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Generation of Immature, Mature and Tolerogenic Dendritic Cells with Differing Metabolic Phenotypes

Published on: June 22, 2016

Human peripheral blood contains two distinct lineages of dendritic cells

S P Robinson1, S Patterson, N English

  • 1Antigen Presentation Research Group, Imperial College School of Medicine at St Mary's, Northwick Park Institute for Medical Research, Harrow, GB.

European Journal of Immunology
|October 3, 1999
PubMed

Insights

Human peripheral blood contains two distinct dendritic cell (DC) lineages. These distinct cell populations differ in markers, antigen uptake, and differentiation potential, suggesting separate developmental origins.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Human peripheral blood contains two distinct populations of dendritic cells (DCs).
  • The developmental relationship between these DC populations remains unclear.

Purpose of the Study:

  • To investigate the developmental relationship between CD11c- and CD11c+ dendritic cells.
  • To characterize the distinct phenotypes and functions of these two DC populations.

Main Methods:

  • Isolation and phenotypic characterization of CD11c- and CD11c+ DCs from human peripheral blood.
  • In vitro culture of DCs under various conditions.
  • Analysis of cell morphology, marker expression (including lymphoid and myeloid markers), antigen uptake, and differentiation potential.
  • Assessment of macrophage colony-stimulating factor (M-CSF) receptor expression and differentiation into macrophages.

Main Results:

  • CD11c- DCs exhibited lymphoid morphology and markers (CD4+, CD10+), while CD11c+ DCs showed monocytoid appearance and myeloid markers.
  • Both DC populations were allostimulatory, but only CD11c+ DCs could uptake antigen.
  • CD11c- cells differentiated into CD11c-, CD13-, CD33-, CD4+, CD1a-, CD83+/- DCs.
  • CD11c+ cells differentiated into CD11c+, CD13+, CD33+/-, CD4-, CD1a+, CD83+, CD9+ DCs.
  • Only CD11c+ DCs expressed the M-CSF receptor and differentiated into CD14+, esterase+, phagocytic macrophages.

Conclusions:

  • The findings suggest that CD11c- and CD11c+ dendritic cells represent distinct lineages of antigen-presenting cells.
  • These distinct lineages possess unique developmental pathways and functional capabilities.

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