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Updated: Aug 8, 2026

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
10:31

Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture

Published on: April 8, 2016

Recent physiopathological insights in cutaneous lymphocytic infiltrates

H Bachelez1

  • 1Institut de Recherche sur la Peau et Service de Dermatologie, Hôpital Saint-Louis, 1, avenue Claude-Vellefaux, 75475 Paris Cedex 10, France. h-bachelez@chu-stlouis.fr

European Journal of Dermatology : EJD
|October 19, 1999
PubMed

Insights

Benign cutaneous lymphocytic infiltrates (CLI) are classified by clinicopathology and T-cell phenotype. Recent studies reveal clonal expansions and antigen-driven mechanisms in HIV-associated pseudolymphomas, improving CLI understanding.

Area of Science:

  • Dermatology
  • Immunology
  • Pathology

Background:

  • Benign cutaneous lymphocytic infiltrates (CLI) encompass diverse entities.
  • Classification relies on clinicopathological findings and lymphocytic subset phenotypes.
  • Recent focus on clonality status using PCR amplification of TCR/Ig loci.

Purpose of the Study:

  • To characterize clonal benign cutaneous lymphocytic expansions.
  • To investigate oligoclonal patterns in HIV-associated CD8 cutaneous pseudolymphomas.
  • To understand the role of antigen-driven mechanisms in pseudolymphoma pathogenesis.

Main Methods:

  • Highly sensitive assays utilizing PCR amplification of TCR/Ig loci.
  • Phenotypic analysis of lymphocytic subsets within skin infiltrates.
  • Functional studies of skin-infiltrating lymphocytes.

Main Results:

  • Characterization of clonal benign cutaneous lymphocytic expansions.
  • Evidence of oligoclonal patterns in HIV-associated CD8 cutaneous pseudolymphomas.
  • Identification of antigen-driven mechanisms in pseudolymphoma pathogenesis.

Conclusions:

  • Clonality assessment enhances understanding of benign CLI.
  • Antigen-driven mechanisms are implicated in HIV-associated pseudolymphomas.
  • Cutaneous Lymphocyte-associated Antigen (CLA) aids in identifying skin-homing T cells.

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