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Determination of LD-Ig complex with counter immunoelectrophoresis using terylene cellulose acetate membrane as
Insights
A new counter immunoelectrophoresis method effectively identifies abnormal lactate dehydrogenase (LD) isoenzyme bands in serum. This technique, using terylene cellulose acetate, is simple and suitable for routine clinical diagnosis of LD anomalies.
Area of Science:
- Biochemistry
- Clinical Chemistry
Background:
- Lactate dehydrogenase (LD) isoenzymes play crucial roles in cellular metabolism.
- Identifying abnormal LD isoenzyme bands in serum is important for diagnosing various medical conditions.
- Existing methods for LD isoenzyme analysis can be complex and time-consuming.
Purpose of the Study:
- To establish a simple and efficient method for determining abnormal LD isoenzyme bands in serum.
- To identify LD-Ig complexes using counter immunoelectrophoresis.
- To evaluate the utility of terylene cellulose acetate as a supporting medium for LD isoenzyme analysis.
Main Methods:
- Serum samples were analyzed using isoenzyme electrophoresis with a Paragon electrophoretic system.
- Counter immunoelectrophoresis with terylene cellulose acetate as the supporting medium was employed to identify LD anomalies.
- The method focused on the identification of the LD-Ig complex.
Main Results:
- An abnormal LD-4 band was detected in a post-burn patient's serum.
- An additional band, identified as an LD-IgG complex, was observed on the cathodic side of LD-5.
- The counter immunoelectrophoresis method proved effective in identifying these abnormal bands.
Conclusions:
- A useful and efficient protocol combining isoenzyme electrophoresis and counter immunoelectrophoresis was developed.
- The described counter immunoelectrophoresis method using terylene cellulose acetate is simple and practical.
- This method is suitable for routine clinical application in identifying abnormal isoenzyme bands.
Objective:
In order to determine abnormal LD isoenzyme bands in serum, we established a method of counter immunoelectrophoresis using terylene cellulose acetate as supporting media.
Setting:
Department of Clinical Laboratory, Southwestern Hospital, Chongqing 400038 China.
Practice Description:
LD isoenzymes were electrophoretically fractionated with a Paragon electrophoretic system. LD anomaly was identified by counter immunoelectrophoresis using terylene cellulose acetate as supporting media.
Main Outcome Measurements:
Identification of the LD-Ig complex using a simple and practical method.
Results:
An abnormal LD-4 band and an extra band on the cathodic side of LD-5 were observed in the serum of a post-burn patient and proved to be an LD-IgG complex.
Conclusion:
We have established a useful and efficient protocol that uses both isoenzyme electrophoresis and counter immunoelectrophoresis to identify abnormal isoenzyme bands. The method of counter immunoelectrophoresis we describe, using terylene cellulose acetate as supporting media, is simple and suitable for routine clinical use.
