Related Experiment Video

Updated: Oct 13, 2026

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

Published on: November 11, 2014

Myb is required for self-renewal in a model system of early hematopoiesis

J R White1, K Weston

  • 1CRC Centre for Cell and Molecular Biology, Institute of Cancer Research, London SW3 6JB, UK.

Oncogene
|March 14, 2000
PubMed

Insights

Myb proteins are crucial for regulating cell fate decisions in hematopoiesis. Inhibiting Myb disrupts self-renewal, promoting apoptosis and differentiation, impacting cell proliferation and survival.

Area of Science:

  • Hematopoiesis
  • Cell Biology
  • Molecular Biology

Background:

  • Hematopoiesis involves critical cell fate decisions: self-renewal, proliferation, differentiation, and apoptosis.
  • Dysregulation of these processes can lead to leukemia.
  • Myb proteins are investigated for their role in regulating these decisions.

Purpose of the Study:

  • To investigate the function of Myb proteins in regulating cell fate decisions during early hematopoiesis.
  • To understand the impact of Myb inhibition on self-renewal, differentiation, and apoptosis in hematopoietic progenitor cells.

Main Methods:

  • Utilized the FDCP-mix A4 cell line as a model for early hematopoiesis.
  • Employed an inducible dominant interfering protein to inhibit endogenous Myb activity.
  • Analyzed cell cycle progression (G1-S phase block) and cell fate outcomes.

Main Results:

  • Inhibition of Myb activity shifted cell fate from self-renewal to apoptosis and differentiation.
  • Differentiation occurred towards granulocytes and monocyte/macrophages, associated with a G1-S phase cell cycle block.
  • Myb inhibition reduced proliferation and survival during cytokine-induced differentiation, lowering mature cell yield.

Conclusions:

  • Myb proteins play multiple critical roles in normal hematopoiesis.
  • Myb is essential for maintaining self-renewal and promoting proliferation and survival during differentiation.
  • Dysregulation of Myb function has significant implications for hematopoietic cell fate and potential leukemogenesis.

More Related Videos

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture09:32

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Published on: May 8, 2018

Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia06:33

Identifying Bone Marrow Microenvironmental Populations in Myelodysplastic Syndrome and Acute Myeloid Leukemia

Published on: November 10, 2023

Related Concept Videos

Hematopoiesis01:21

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...