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Published on: July 10, 2014
[The evaluation of noninvasive samples for detecting anti-cytomegalovirus IgG antibodies]
1Disciplina de Microbiologie, Facultatea de Medicină, Universitatea de Medicină şi Farmacie Gr. T. Popa, Iaşi.
Insights
Cytomegalovirus (CMV) infection is common in dialysis patients. Non-invasive saliva and capillary blood tests are effective for CMV IgG antibody surveillance in this high-risk group.
Area of Science:
- Virology
- Infectious Diseases
- Public Health
Background:
- Dialysis patients face high risks for blood-borne infections.
- Cytomegalovirus (CMV) infection is a frequent concern, often transmitted via transfused blood products.
Purpose of the Study:
- To determine the prevalence of IgG anti-CMV antibodies in dialysis patients.
- To evaluate the utility of non-invasive samples (saliva, capillary blood) for epidemiological studies of CMV infection.
Main Methods:
- ELISA Wellcome anti-CMV IgG assay used for antibody detection.
- Samples analyzed: serum, saliva, and capillary blood.
- Comparison of non-invasive sample results against serum results for specificity, sensitivity, and predictive values.
Main Results:
- Prevalence of anti-CMV IgG in the study group exceeded general population levels.
- High positivity rates observed: 79.06% in saliva and 93.76% in capillary blood.
- Non-invasive samples demonstrated utility for epidemiological surveillance.
Conclusions:
- Non-invasive samples (saliva, capillary blood) are valuable tools for CMV infection surveillance in high-risk patient groups.
- ELISA testing on these samples provides reliable data for epidemiological studies.
- This approach aids in monitoring CMV in vulnerable populations like dialysis patients.
Abstract:
Patients admitted in dialysis centers are at greatest risk to achieve blood-borne infections. One of the most frequent is the cytomegalovirus infection, acquired from transfused blood products. The aim of our study was to determine the prevalence of IgG anti-CMV in patients on dialysis at the "C. I. Parhon" Hospital from Iaşi (Romania). In addition, the role of non-invasive samples (saliva and capillary blood) for such epidemiological studies was determined. ELISA Wellcome anti-CMV IgG was used for identification of specific antibodies in serum, capillary blood, saliva. The prevalence of anti-CMV IgG in the group of study was higher than that established in general populations and 79.06% saliva specimens and 93.76% of capillary blood samples were positive. To establish the specificity, sensitivity and predictive values of Wellcome anti-CMV when non-invasive samples are used, we compared the results obtained by testing saliva and capillary blood to those obtained by testing serum. We may conclude that the non-invasive samples are useful for CMV-infection surveillance in risk groups.
