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Updated: Aug 11, 2026
Immunodetection of Outer Membrane Proteins by Flow Cytometry of Isolated Mitochondria
Published on: September 18, 2014
Detection of intracytoplasmic cytokines by flow cytometry
M A Santiago1, P M Luca, A L Bertho
1Laboratório de Imunidade Celular e Humoral em Protozooses, Departamento de Protozoologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil. santiago@gene.dbbm.fiocruz.br
Insights
Flow cytometry is a powerful tool for analyzing cells, enabling the study of cell surface and intracellular molecules. New techniques now allow detailed analysis of cytokine-producing cells in humans and animals.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Background:
- Flow cytometry is a key technique for analyzing cell surface and intracellular molecules.
- It enables the simultaneous multi-parameter analysis of single cells within heterogeneous populations.
- This facilitates the identification and functional study of specific cell subsets.
Purpose of the Study:
- To review recent advancements in flow cytometry techniques.
- To highlight methods for studying cytokine-producing cells.
- To cover applications in human and animal models.
Main Methods:
- Review of established and novel flow cytometry protocols.
- Focus on intracellular cytokine staining techniques.
- Application in diverse biological systems.
Main Results:
- Flow cytometry allows detailed characterization of cytokine-producing cell populations.
- Multiple techniques have been refined for studying cytokine expression.
- These methods are applicable across various species.
Conclusions:
- Flow cytometry is instrumental in dissecting immune responses.
- Advanced techniques enhance the study of cytokine-producing cells.
- This technology is vital for research in human and veterinary immunology.
Abstract:
Flow cytometry has been used as a powerful technique for studying cell surface antigen expression as well as intracellular molecules. Its capability of analyzing multiple parameters simultaneously on a single cell has allowed identification and studies of functional cell subsets within heterogeneous populations. In this respect, several techniques have been developed during the past few years to study cytokine-producing cells by flow cytometry in humans and several animal models.
