Related Experiment Video

Updated: Aug 8, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
12:52

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

BLNK is associated with the CD72/SHP-1/Grb2 complex in the WEHI231 cell line after membrane IgM cross-linking

N Fusaki1, S Tomita, Y Wu

  • 1Research Institute for Biological Sciences, Science University of Tokyo, Noda, Chiba, Japan.

European Journal of Immunology
|May 23, 2000
PubMed

Insights

Tyrosine phosphorylation of CD72 links B cell receptor signals to apoptosis. This study reveals BLNK associates with the CD72 complex, involving SHP-1 and Grb2, during B cell apoptosis.

Area of Science:

  • Immunology
  • Cell Signaling
  • Molecular Biology

Background:

  • CD72 tyrosine phosphorylation correlates with B cell antigen receptor (BCR) signals and apoptosis.
  • CD72 contains immunoreceptor tyrosine-based inhibition motifs (ITIMs) and is a substrate of SHP-1.
  • CD72 forms a complex with SHP-1 and Grb2 in apoptotic immature B cells (WEHI231 cell line) and normal primary B cells.

Purpose of the Study:

  • To investigate the molecular components of the CD72 complex in WEHI231 cells.
  • To elucidate the molecular mechanism of the signaling pathway mediated through the CD72 complex.
  • To understand the role of BLNK in the CD72 complex.

Main Methods:

  • Utilized the WEHI231 cell line, representative of immature B cells.
  • Investigated protein-protein interactions within the CD72 complex.
  • Analyzed complex formation after membrane IgM (mIgM) engagement.

Main Results:

  • BLNK (B cell linker protein), an adaptor molecule, associates with the CD72 complex.
  • Association of BLNK with the CD72 complex occurs via the Src homology 3 domain(s) of Grb2.
  • The mIgM-mediated signal correlates with the formation of the CD72 / SHP-1 / Grb2 / BLNK complex.

Conclusions:

  • BLNK is a key molecular component of the CD72 signaling complex in B cells.
  • The formation of the CD72 / SHP-1 / Grb2 / BLNK complex is a critical event in mIgM-mediated signaling leading to B cell apoptosis.
  • This complex formation represents a conserved mechanism in B cell signaling.

More Related Videos

A BW Reporter System for Studying Receptor-Ligand Interactions06:05

A BW Reporter System for Studying Receptor-Ligand Interactions

Published on: January 7, 2019

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...