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CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression

P Kirk1, M C Wilson, C Heddle

  • 1Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, UK.

The EMBO Journal
|August 2, 2000
PubMed

Insights

Cluster of Differentiation 147 (CD147) protein directly interacts with monocarboxylate transporters (MCTs) 1 and 4. This interaction is crucial for MCT1 and MCT4 proper cell surface expression and function in cellular metabolism.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • CD147 is a plasma membrane glycoprotein with immunoglobulin-like domains.
  • Monocarboxylate transporters (MCTs) are vital for cellular metabolism, particularly lactate transport.
  • The precise role of CD147 in regulating MCT function is not fully understood.

Purpose of the Study:

  • To investigate the interaction between CD147 and members of the MCT family.
  • To determine the domains of CD147 involved in MCT binding.
  • To elucidate the functional consequences of CD147-MCT interaction on transporter localization and activity.

Main Methods:

  • Co-immunoprecipitation and chemical cross-linking assays were employed.
  • Studies utilized a CD2-CD147 chimera to map interaction domains.
  • Immunofluorescence microscopy and cell transfection experiments were performed.

Main Results:

  • CD147 specifically binds to MCT1 and MCT4, but not MCT2.
  • The transmembrane and cytoplasmic domains of CD147 mediate the interaction.
  • CD147 co-localizes with MCT1 in heart cells and facilitates plasma membrane expression of MCT1 and MCT4.

Conclusions:

  • CD147 acts as a crucial chaperone for the cell surface expression of MCT1 and MCT4.
  • The interaction between CD147 and MCTs is essential for their proper localization and potentially their activity.
  • This finding provides new insights into the regulation of cellular metabolism and lactate transport.

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