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Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Shipment impairs lymphocyte proliferative responses to microbial antigens

R A Betensky1, E Connick, J Devers

  • 1Harvard School of Public Health, Boston, Massachusetts, USA.

Clinical and Diagnostic Laboratory Immunology
|September 6, 2000
PubMed

Insights

Shipping lymphocyte proliferation assays (LPAs) significantly reduces T-lymphocyte function activity. This impacts accuracy for immunodeficiency disorder testing and multicenter trials.

Area of Science:

  • Immunology
  • Clinical Diagnostics

Background:

  • Lymphocyte proliferation assays (LPAs) are crucial for evaluating T-lymphocyte function in immunodeficiency disorders.
  • Clinical sites often lack specialized expertise, necessitating specimen shipment to central laboratories.

Purpose of the Study:

  • To assess the impact of shipping on the performance and reliability of LPAs.
  • To identify potential biases introduced in multicenter studies due to specimen transport.

Main Methods:

  • A large-scale, multicenter study was designed to evaluate LPA performance.
  • Specimens were shipped from various clinical sites to central laboratories for analysis.

Main Results:

  • Significant reductions in LPA activity were observed after specimen shipping.
  • Shipping demonstrably affected the reliability of T-lymphocyte function assessment.

Conclusions:

  • Specimen shipping can lead to erroneous results in individual patient assessments.
  • The observed loss of LPA activity poses a risk of bias in multicenter clinical trials.

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