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Soluble CD86 is a costimulatory molecule for human T lymphocytes

P Jeannin1, G Magistrelli, J P Aubry

  • 1Centre d'Immunologie Pierre Fabre, Saint Julien en Genevois, France.

Immunity
|October 6, 2000
PubMed

Insights

Soluble CD86, a functional costimulatory molecule, is found in human serum. It is produced by monocytes and can activate T cells, suggesting a role in immune surveillance.

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • CD86 is a key costimulatory molecule essential for T cell activation.
  • Its role in soluble form and its presence in human serum were previously uncharacterized.

Purpose of the Study:

  • To investigate the presence and function of soluble CD86 in human serum.
  • To characterize the molecular nature and biological activity of soluble CD86.

Main Methods:

  • Detection of soluble CD86 in human serum.
  • Analysis of alternatively spliced CD86 transcripts (CD86deltaTM).
  • Recombinant CD86deltaTM binding assays with CD28 and CTLA-4.
  • T cell activation assays using anti-CD3 mAb and Flu M1 peptide.

Main Results:

  • Soluble CD86 was detected in human serum, originating from resting monocytes.
  • An alternatively spliced transcript, CD86deltaTM, lacking the transmembrane domain, was identified as the source.
  • Recombinant CD86deltaTM demonstrated binding to CD28 and CTLA-4.
  • Soluble CD86 induced T cell activation and IFNgamma production in virus-specific memory T cells.

Conclusions:

  • Soluble CD86 is a biologically active molecule present in human serum at functional concentrations.
  • This soluble form of CD86 may play a significant role in immune surveillance.

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