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[Interference in turbidimetric immunoassay for serum C-reactive protein due to serum protein abnormalities an immune

T Ohtake1, S Kano, K Watanabe

  • 1Laboratory Medicine, Keio University School of Medicine, Tokyo.

Rinsho Byori. the Japanese Journal of Clinical Pathology
|November 7, 2000
PubMed

Insights

Serum protein abnormalities can interfere with C-reactive protein (CRP) immunoassays. Strategies like serum predilution and modified latex binding effectively reduce interference in turbidimetric and latex photometric immunoassay methods.

Area of Science:

  • Clinical Chemistry
  • Immunology
  • Biochemistry

Background:

  • Immunoassays for C-reactive protein (CRP) are susceptible to interference from abnormal serum proteins.
  • Turbidimetric immunoassay (TIA) and latex photometric immunoassay (LPIA) are common methods for CRP detection.

Purpose of the Study:

  • To investigate serum protein abnormalities that interfere with CRP immunoassays.
  • To evaluate methods for mitigating interference in TIA and LPIA.

Main Methods:

  • Studied interference from immune complexes, agglutinating immunoglobulins, and rheumatoid factor (RF).
  • Examined two cases of TIA interference (paraproteinemia, cryoglobulinemia) and one case of LPIA interference (RF).
  • Assessed serum predilution and covalent binding of IgG(Fab')2 to latex particles as mitigation strategies.

Main Results:

  • Immune complexes and agglutinating immunoglobulins caused falsely low CRP values in TIA.
  • Rheumatoid factor led to falsely high CRP values in TIA and LPIA.
  • Serum predilution effectively reduced interference in TIA for specific cases.
  • Covalent IgG(Fab')2 binding eliminated RF interference in LPIA.

Conclusions:

  • Serum protein abnormalities pose a significant challenge in accurate CRP quantification.
  • Serum predilution and optimized immunoassay reagent preparation are crucial for reliable CRP results.

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