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Comparative CD43 behavior on monocytes and lymphocytes in kidney transplants
Insights
CD43 dys-sialylation, a modification of sialic acid epitopes, is observed in kidney transplant recipients (KTR) monocytes. This alteration, potentially linked to cyclosporin, may affect monocyte function and adhesion.
Area of Science:
- Immunology
- Cell Biology
- Transplantation Science
Background:
- CD43 exhibits sialylation modifications in cytokine-stimulated human monocytic cells.
- Monocytes play critical roles in immune responses and post-transplant complications.
Purpose of the Study:
- To investigate CD43 expression and sialylation status on monocytes from kidney transplant recipients (KTR).
- To explore the potential role of cyclosporin (CsA) in CD43 dys-sialylation in KTR.
Main Methods:
- Flow cytometry using monoclonal antibodies targeting sialic acid-dependent (L60) and -independent (L10) CD43 epitopes.
- Analysis of CD43 expression on monocytes and lymphocytes from KTR and healthy controls.
- In vitro experiments using THP-1 (monocytic) and Jurkat (lymphoid) cell lines treated with CsA.
Main Results:
- KTR monocytes showed altered CD43 expression profiles, with decreased L60 staining in 54% and a double population in 20% of patients.
- Decreased CD43 sialylation (dys-sialylation) was more prevalent in KTR within 3 months post-transplantation.
- L10 epitope expression remained unaltered, confirming dys-sialylation, and CsA treatment reduced CD43 expression in monocytic cells but not lymphoid cells.
Conclusions:
- Kidney transplant recipients exhibit CD43 dys-sialylation on monocytes, particularly in the early post-transplant period.
- Cyclosporin may contribute to this dys-sialylation, potentially impacting monocyte adhesion and function in KTR.
Abstract:
In human cultured monocytic cells stimulated by cytokines, CD43 was demonstrated to exhibit a modification of sialylated epitopes (dys-sialylation) [Soler et al: Leukoc Biol 1997;61:609-618]. Therefore, we chose to investigate CD43 behavior on patients who present pathological status implicating monocytes after renal graft (KTR). We performed flow cytometry after immune staining using monoclonal antibodies to CD43 sialic acid-dependent (L60) and -independent (L10) epitopes. Compared to normal controls, mean fluorescence intensity was never altered on lymphocytes. Conversely, on monocytes, we found different profiles with L60: 26% of patients having normal CD43 expression, 54% displayed decreased values and 20% had a double population of monocytes, the major one being normal and the minor one with a very low staining. Decreased values were more frequent among KTR during the first 3 months following transplantation. L10 immunostaining was not altered on monocytes in patients with low values of CD43 staining by L60, confirming that the mechanism involved was a CD43 dys-sialylation. We investigated a possible role of cyclosporin (CsA) on human monocytic (THP-1) and lymphoid (Jurkat) cell lines. CsA decreases CD43 expression in monocytic and not in lymphoid cell lines and could be responsible for the specific dys-sialylation of KTR monocytes. Whatever, CD43 dys-sialylation might lead to functional abnormalities of monocytes in KTR, possibly involving the adhesion process.