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Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
CD1a+, CD3+, CD4+, CD8+, CD68+ and cutaneous lymphocyte-associated antigen-positive cells in Bowen's disease
1Department of Dermatology, Graduate School of Medical Sciences, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812-8582, Japan.
Insights
Bowen's disease (BD) shows increased cell proliferation and altered immune cell presence. Dermal CD1a+ cells may aid immune surveillance, while HPV infection may hinder immune cell infiltration in BD.
Area of Science:
- Dermatology
- Immunohistology
- Oncology
Background:
- Bowen's disease (BD) is a squamous cell carcinoma in situ with limited immunohistological understanding.
- Investigating the interplay between tumor cell characteristics and host immune response in BD is crucial.
Purpose of the Study:
- To evaluate the relationship between cytological properties of tumor cells in BD and the host immune response.
- To assess the expression of key cellular markers and immune cell infiltration in genital BD.
Main Methods:
- Examined p53, PCNA, Ki67 expression, and mitotic cell counts in 18 genital BD cases.
- Quantified intratumoral and dermal CD1a+, CD3+, CD4+, CD8+, CD68+, and CLA+ cells.
- Utilized in situ hybridization for human papillomavirus (HPV) detection.
Main Results:
- BD cases showed higher mitotic activity and p53, PCNA, Ki67 expression than normal skin.
- Significant correlations were observed between CD3+, CD4+, and CD68+ cells in tumoral epidermis.
- CD1a+ Langerhans cells decreased in BD epidermis but increased in the dermis, correlating with intratumoral immune cells.
Conclusions:
- Dermal CD1a+ cells may play a role in immune surveillance in BD.
- HPV infection appears to impede intratumoral infiltration of CLA+ cells in BD.
Background:
Bowen's disease (BD) is a squamous cell carcinoma in situ that rarely invades into the underlying dermis. However, little is known about its immunohistology. Objectives To evaluate the relationship between the cytological properties of the tumour cells in BD and the host immune response.
Methods:
We examined the expression of p53, proliferating cell nuclear antigen (PCNA) and Ki67 antigen, and the number of mitotic cells, together with the number of intratumoral and dermal infiltrating CD1a+, CD3+, CD4+, CD8+, CD68+ and cutaneous lymphocyte-associated antigen (CLA)+ cells in 18 cases of genital BD.
Results:
When compared with normal genital skin (n = 10), there was a significantly higher number of mitotic cells as well as higher expression of p53+, PCNA+ and Ki67+ cells in BD. There was significant mutual correlation between CD3+, CD4+ and CD68+ cells in the tumoral epidermis. The number of CD1a+ Langerhans cells significantly decreased in BD epidermis; however, dermal CD1a+ cells were increased. Interestingly, numbers of dermal CD1a+ cells significantly correlated with those of intratumoral CD3+, CD4+ and CD68+ cells. In situ hybridization for human papillomavirus (HPV) demonstrated that HPV-infected BD had significantly less infiltration of intratumoral CD3+ cells and CLA+ cells.
Conclusions:
The present data suggest that dermal CD1a+ cells may participate in the immune surveillance and that HPV infection may interfere with the intratumoral infiltration of CLA+ cells in BD.
