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Analysis of the Epithelial Damage Produced by Entamoeba histolytica Infection
Published on: June 12, 2014
Experimental amebiasis: immunohistochemical study of immune cell populations
P K Ghosh1, G J Ventura, S Gupta
1Department of Immunology, Instituto de Investigaciones Biomedicas, UNAM, Mexico DF.
Insights
Immune cell distribution in intestinal amebiasis was analyzed in mice. IgA-producing plasma cells dominated the mucosal immune response, suggesting their importance in combating Entamoeba histolytica infection.
Area of Science:
- Immunology
- Parasitology
- Gastroenterology
Background:
- Intestinal amebiasis, caused by Entamoeba histolytica, is a significant global health concern.
- Understanding the host immune response is crucial for developing effective treatments.
Purpose of the Study:
- To investigate the distribution and types of immune cells in the mouse cecum during intestinal amebiasis.
- To identify key immune players involved in the host defense against Entamoeba histolytica.
Main Methods:
- Utilized a C3H/HeJ mouse model of intestinal amebiasis.
- Employed immunoperoxidase techniques to detect IgA, IgM, IgG, CD3, CD4, CD8, CD11 cells, neutrophils, eosinophils, and Mast cells.
- Analyzed immune cell populations from 5 to 60 days post-inoculation.
Main Results:
- IgA-producing plasma cells were the predominant immune cells in the mucosa.
- Significant infiltration of eosinophils, neutrophils, CD3+, CD4+, CD8+, IgM+, IgG+ cells, and Mast cells was observed.
- Immune cell presence varied throughout the 60-day infection period.
Conclusions:
- The study highlights the dominance of IgA+ plasma cells in the mucosal immune response to intestinal amebiasis.
- Immune cell infiltration at the mucosal surface appears critical for defense against Entamoeba histolytica.
- Further research into these immune mechanisms could lead to novel therapeutic strategies.
Abstract:
Distribution of immune cell populations was studied in a C3H/HeJ mouse model of intestinal amebiasis from 5 to 60 days post-inoculation with Entamoeba histolytica, using immunoperoxidase techniques. At various time intervals, the ceca from mice were fixed in 10% formalin, dehydrated, embedded and sectioned at 5 microm. Sections were incubated with conjugated peroxidase-labelled antibodies to mouse IgA, IgM, and IgG. Color was developed with 3, 3'-diaminobenzidine tetrahydrochloride (DAB)/H2O2 solution. CD3, CD4, and CD8 cells, as well as neutrophils were detected by reacting with biotin-conjugated anti-mouse CD3, CD4, CD8, and CD11 monoclonal antibodies, followed by their incubation with avidin-peroxidase and color development with DAB/H2O2 solution. Erythrocin B and toluidine blue were used to stain eosinophils and Mast cells, respectively. It was observed that the IgA+ plasma cell was the dominating immune cell present in the mucosa, although eosinophils, neutrophils, CD3+, CD4+, CD8+, IgM+, IgG+ cells and Mast cells were also seen. Results of this study suggest that infiltration of immune cells at the mucosal surface during intestinal amebiasis might be important in the defense against this parasite.
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