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The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo
12:09

The Use of Fluorescent Target Arrays for Assessment of T Cell Responses In vivo

Published on: June 19, 2014

Immunofluorescence analysis of T-cell responses in health and disease

H T Maecker1, V C Maino, L J Picker

  • 1BD Immunocytometry Systems, San Jose, California 95131, USA.

Journal of Clinical Immunology
|February 24, 2001
PubMed

Insights

Flow cytometry using immunofluorescent staining rapidly evaluates T-cell functional responses to mitogens and antigens. These methods show promise for diverse clinical applications, enhancing T-cell analysis.

Area of Science:

  • Immunology
  • Cellular Biology
  • Clinical Diagnostics

Background:

  • Flow cytometry is increasingly utilized for studying T-cell functional responses.
  • Immunofluorescent staining for intracellular cytokines aids in T-cell subset analysis.
  • Assessing T-cell function is crucial for understanding immune status.

Purpose of the Study:

  • To describe methods for rapid T-cell response evaluation using flow cytometry.
  • To highlight the utility of these assays in clinical settings.
  • To explore the application of intracellular cytokine staining in T-cell research.

Main Methods:

  • Utilizing flow cytometry for high-throughput analysis of T-cells.
  • Employing immunofluorescent staining for intracellular cytokine detection.
  • Testing T-cell responses to specific antigens and mitogens.

Main Results:

  • Demonstrated rapid and effective evaluation of T-cell functional responses.
  • Showcased the versatility of flow cytometry in clinical immunology.
  • Provided a framework for assessing T-cell activation and cytokine production.

Conclusions:

  • Flow cytometry with intracellular cytokine staining offers a valuable tool for clinical T-cell analysis.
  • Rapid T-cell response assays can be implemented in various clinical scenarios.
  • This approach enhances the understanding of T-cell-mediated immunity in health and disease.

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