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Modulation of CD8 and CD3 by HIV or HIV antigens

J Jason1, K L Inge

  • 1Mailstop A-25, Immunology Branch, DASTLR/NCID, Centers for Disease Control and Prevention, Department of Health and Human Services, Public Health Service, 1600 Clifton Road NE, Atlanta, GA 30333, USA. JMJ1@cdc.gov

Scandinavian Journal of Immunology
|March 17, 2001
PubMed

Insights

Human immunodeficiency virus (HIV) infection and its components, Nef and Rev, significantly alter CD8 T-cell expression. This modulation of CD8, crucial for immune response, may impact HIV pathogenesis and treatment strategies.

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Background:

  • Human immunodeficiency virus (HIV) infection impacts immune cell function.
  • Surface and cytoplasmic CD markers (CD3, CD4, CD8) are critical for T-cell activity.
  • Understanding HIV's effect on these markers is key to comprehending pathogenesis.

Purpose of the Study:

  • To investigate how HIV-1 and its antigens (gp120, Rev, Nef) modulate surface and cytoplasmic CD3, CD4, and CD8 expression.
  • To determine if HIV-1 infection or its components directly affect T-cell marker expression.

Main Methods:

  • Utilized in vitro culture systems with human peripheral blood lymphocytes.
  • Employed cell permeabilization, surface/cytoplasmic fluorescent staining, and multiparameter flow cytometry.
  • Compared unstimulated, HIV-inoculated, PHA-stimulated, PHA/HIV-inoculated, and cells treated with gp120, Rev, or Nef.

Main Results:

  • HIV inoculation and Nef significantly modulated CD8 expression, reducing cytoplasmic CD8 MFI in infected cells.
  • Nef treatment led to significantly lower surface CD8 MFI.
  • Rev exposure showed reductions in surface CD4 MFI and notable effects on cytoplasmic CD3 and surface CD8 MFI.

Conclusions:

  • HIV, Nef, and Rev demonstrably alter CD3, CD4, and CD8 expression on lymphocytes.
  • Down-modulation of CD8 by HIV and Nef may impair cytotoxic T-cell anti-HIV activity.
  • These findings have significant clinical implications for HIV pathogenesis and therapeutic interventions.

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