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An Ex Vivo Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
Published on: October 4, 2018
Detection of cell membrane proteins that interact with virulent infectious bursal disease virus
A Setiyono1, T Hayashi, T Yamaguchi
1Department of Veterinary Microbiology, Faculty of Agriculture, Gifu University, Japan.
Insights
Researchers identified specific cellular molecules that interact with the infectious bursal disease virus (IBDV). This study reveals key protein targets for understanding virulent IBDV infection in chickens.
Area of Science:
- Veterinary Virology
- Cellular Biology
- Immunology
Background:
- Infectious bursal disease virus (IBDV) is a highly contagious pathogen affecting poultry worldwide.
- Understanding IBDV-host interactions is crucial for developing effective control strategies.
- The specific cellular molecules targeted by virulent IBDV remain largely unidentified.
Purpose of the Study:
- To identify cellular molecules that interact with virulent infectious bursal disease virus (IBDV).
- To investigate the binding of IBDV to proteins in a chicken B lymphoblastoid cell line.
Main Methods:
- Utilized the LSCC-BK3 chicken B lymphoblastoid cell line, which is permissive to virulent IBDV.
- Employed a virus overlay protein binding assay on sodium dodecyl sulfate-solubilized plasma membrane fractions.
- Analyzed protein binding based on molecular weights.
Main Results:
- IBDV specifically bound to proteins in LSCC-BK3 plasma membranes.
- The identified interacting proteins had molecular weights of 70, 82, and 110 kDa.
- This is the first report demonstrating cellular molecules that interact with virulent IBDV.
Conclusions:
- Specific cellular proteins of 70, 82, and 110 kDa are involved in virulent IBDV interaction.
- These findings provide a foundation for further research into IBDV pathogenesis and host-pathogen interactions.
Abstract:
To detect the molecules that interact with infectious bursal disease virus (IBDV), the chicken B lymphoblastoid cell line, LSCC-BK3, which is permissive for virulent IBDV infection was investigated. The sodium dodecyl sulfate-solubilized plasma membrane fraction from the cells was subjected to a virus overlay protein binding assay. The IBDV specifically bound to proteins in LSCC-BK3 plasma membranes with molecular weights of 70, 82 and 110 kDa. This is the first report to demonstrate cellular molecules that interact with virulent IBDV.
