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Activation-induced expression of CD1d antigen on mature T cells

M C Salamone1, G A Rabinovich, A K Mendiguren

  • 1Division Inmunogenética, Hospital de Clínicas, Facultad de Medicina, Universidad de Buenos Aires, Argentina. marysasinec@is.com.ar

Journal of Leukocyte Biology
|March 29, 2001
PubMed

Insights

Human CD1d antigen expression increases on activated T cells, indicating its role in immune regulation. This study reveals CD1d

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • CD1d is a nonclassical MHC class I molecule involved in T cell activation.
  • The expression patterns and regulation of CD1d on T cells are not fully understood.

Purpose of the Study:

  • To investigate the expression of human CD1d antigen on activated mature T cells.
  • To determine if CD1d expression is regulated by T cell activation.

Main Methods:

  • Flow cytometry using anti-CD1d antibody (NOR3.2).
  • Reverse transcription polymerase chain reaction (RT-PCR) for CD1d mRNA.
  • Western blot analysis for CD1d protein.
  • Immunocytochemistry for protein localization.

Main Results:

  • PHA stimulation significantly increased CD1d expression on T cells.
  • CD1d mRNA and protein levels were upregulated upon T cell activation.
  • CD1d was localized to the plasma membrane, cytoplasm, and perinuclear region.
  • Activation-dependent CD1d expression was observed in both PHA- and allogeneic-activated T cells.

Conclusions:

  • CD1d antigen is present on circulating activated T lymphocytes.
  • CD1d expression is highly regulated and dependent on the activation state of T cells.
  • These findings suggest a potential role for CD1d in T cell-mediated immune responses and regulation.

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