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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
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Published on: July 26, 2017

Constitutive and cytokine-induced expression of MHC and intercellular adhesion molecule-1 (ICAM-1) on human myoblasts

M Marino1, F Scuderi, P Mazzarelli

  • 1Institute of General Pathology, Catholic University, Largo Francesco Vito 1, 00168, Rome, Italy.

Journal of Neuroimmunology
|April 20, 2001
PubMed

Insights

Cytokines like IFN-gamma, LPS, and IL-4 significantly boost MHC-I expression in human myoblasts. Muscle adhesion molecule expression is finely tuned by various cytokines, impacting cellular responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Muscle Physiology

Background:

  • Immune cells and cytokines play crucial roles in regulating cellular functions.
  • Understanding the molecular mechanisms of immune responses in muscle cells is vital.

Purpose of the Study:

  • To investigate the impact of key cytokines on the expression of MHC-I, MHC-II, and ICAM-1 in human myoblasts.
  • To elucidate the regulatory roles of specific cytokines in muscle cell immune molecule expression.

Main Methods:

  • Cultured human myoblasts were treated with various cytokines: IL-1beta, IL-4, IL-6, IFN-gamma, and LPS.
  • Expression levels of MHC-I, MHC-II, and ICAM-1 (membrane and soluble forms) were analyzed.
  • mRNA expression of ICAM-1 was also assessed.

Main Results:

  • IFN-gamma, LPS, and IL-4 significantly upregulated MHC-I molecule expression.
  • MHC-II molecule expression was exclusively induced by IFN-gamma.
  • Both membrane and mRNA expression of ICAM-1 were induced by all tested cytokines, with varying efficiencies and time courses.
  • Soluble ICAM-1 secretion was also induced by all cytokines to different extents.

Conclusions:

  • Cytokines exhibit distinct and potent effects on the expression of MHC-I and MHC-II in human myoblasts.
  • Intercellular Adhesion Molecule-1 (ICAM-1) expression and secretion are finely regulated by a range of cytokines in muscle cells.
  • These findings highlight the complex cytokine-mediated regulation of adhesion-related molecules in muscle tissue.

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