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Updated: Aug 10, 2026
Isolation and Characterization of Neutrophils with Anti-Tumor Properties
Published on: June 19, 2015
Immunobiology of CD28 expression on human neutrophils. I. CD28 regulates neutrophil migration by modulating CXCR-1
K Venuprasad1, P Parab, D V Prasad
1National Centre for Cell Science, Ganeshkhind, Pune, India.
Insights
CD28 is expressed on human neutrophils, regulating IL-8 receptor A expression and migration. This finding reveals a novel role for CD28 beyond T cells in neutrophil function.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD28 is a known T cell costimulatory molecule.
- Neutrophils play critical roles in innate immunity and inflammation.
Purpose of the Study:
- To investigate the expression and function of CD28 on human peripheral blood neutrophils.
- To determine CD28's role in regulating neutrophil responses to IL-8.
Main Methods:
- Cell surface staining and immunoprecipitation using anti-CD28 monoclonal antibody.
- Reverse transcription PCR (RT-PCR) for CD28 mRNA detection.
- Analysis of IL-8 receptor A (CXCR-1) expression and IL-8-induced chemotaxis following CD28 cross-linking.
Main Results:
- CD28 is expressed on human peripheral blood neutrophils.
- CD28 expression on neutrophils is regulated by transcription and protein kinase activity.
- CD28 cross-linking increases IL-8 receptor A (CXCR-1) expression and IL-8-induced neutrophil chemotaxis.
- Down-regulation of CXCR-1 and reduced chemotaxis occur 3 hours post-CD28 cross-linking.
Conclusions:
- CD28 is expressed on human neutrophils, expanding its known functions.
- CD28 signaling influences IL-8 receptor A expression and neutrophil migration.
- CD28 may be a key regulator of neutrophil inflammatory responses.
Abstract:
CD28, described as a T cell costimulatory molecule so far, is expressed on human peripheral blood neutrophils, as shown by cell surface staining and immunoprecipitation with anti-CD28 monoclonal antibody, and by reverse transcription PCR. The phorbol 12-myristate 13-acetate-augmented expression of CD28 on these cells can be blocked by actinomycin D, an RNA transcription inhibitor, and staurosporin, a protein kinase inhibitor. Cross-linking of CD28 results in an early increase in IL-8 receptor A (IL-8RA or CXCR-1) expression and a concurrent increase in IL-8-induced chemotaxis. The expression of CXCR-1 is down-regulated by receptor internalization 3 h after CD28 cross-linking with concurrent decrease in IL-8-induced chemotactic migration. Thus, our results demonstrate for the first time that CD28 is expressed on human peripheral blood neutrophils and that CD28 may play an important role in the regulation of IL-8RA expression and migration of neutrophils in response to IL-8.
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