[Study on in vitro cytokines levels induced from peripheral mononuclear cells in patients with endometriosis]
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Published on: March 2, 2017
Insights
Endometriosis patients exhibit disordered immune cell function, with elevated levels of interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), and nitric oxide (NO). These inflammatory markers may contribute to endometriosis development.
Area of Science:
- Immunology
- Reproductive Medicine
- Inflammation Research
Context:
- Endometriosis (Em) is a complex gynecological condition characterized by inflammation.
- Peripheral blood mononuclear cells (PBMCs) play a crucial role in immune responses.
- Understanding the inflammatory milieu in endometriosis is vital for therapeutic development.
Purpose:
- To investigate the levels of key inflammatory cytokines: interleukin-6 (IL-6), interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha), and nitric oxide (NO).
- To analyze these inflammatory markers in vitro using PBMCs from endometriosis patients and healthy controls.
Summary:
- PBMCs were isolated from 31 endometriosis patients (mild and severe stages) and 15 controls.
- Cells were stimulated and incubated, with inflammatory marker levels measured via ELISA and Griess methods.
- Significantly higher concentrations of IL-6, IL-8, TNF-alpha, and NO were observed in endometriosis groups compared to controls, with strong positive correlations among these markers.
Impact:
- The study reveals disordered immune function in endometriosis patients, indicated by elevated inflammatory mediators.
- These findings suggest that activated PBMCs producing high levels of IL-6, IL-8, TNF-alpha, and NO may contribute to the pathogenesis of endometriosis.
- This research provides insights into the inflammatory mechanisms underlying endometriosis, potentially guiding future treatment strategies.
Objective:
To study the changes and significance of interleukin-6 (IL-6), interleukin-8(IL-8), tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) levels in vitro cultured system of peripheral mononuclear cells in patient with endometriosis(Em).
Methods:
Peripheral blood mononuclear cells from 31 cases of endometriosis, classified into mild group (stage I and II, n = 11), and severe group (stage III and IV, n = 20) according to the Revised American Fertility society criteria 1985, and 15 normal controls were separated and induced by lipopolysaccharide and phytohemagglutinin, followed by 48 hour incubation in vitro, then the supernatant were collected. The levels of IL-6, IL-8 and TNF-alpha were measured by enzyme linked immunosorbent assays, the concentrations of NO, expressed by nitrite and nitrate (NO2-/NO3-) content were measured by Griess methods.
Results:
The concentrations of IL-6, IL-8, TNF-alpha and NO were significantly higher in both mild and severe Em groups than those in controls (P < 0.01). No differences between the mild and severe Em groups was seen. There were strong positive correlation between the levels of IL-6 and IL-8, TNF-alpha and NO in patients with endometriosis (P < 0.01).
Conclusions:
The immune function of peripheral mononuclear cell in patients with endometriosis was disordered. It may activate peripheral mononuclear cells to produce high levels of IL-6, IL-8, TNF-alpha and NO, which may take part in the pathogenesis of endometriosis.
