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Published on: October 30, 2012
CD22 regulates B cell receptor-mediated signals via two domains that independently recruit Grb2 and SHP-1
K L Otipoby1, K E Draves, E A Clark
1Department of Immunology, University of Washington, Seattle, Washington 98195, USA. kotipoby@u.washington.edu
Insights
CD22, a B cell coreceptor, recruits signaling molecules like SHP-1 and Grb2 upon B cell antigen receptor (BCR) activation. Distinct tyrosine residues on CD22 regulate these distinct signaling pathways, influencing B cell fate.
Area of Science:
- Immunology
- Cell Signaling
- Molecular Biology
Background:
- B cell antigen receptor (BCR) recognition dictates B cell fate.
- Coreceptors, like CD22, modulate BCR signaling.
- CD22 is a B cell-specific coreceptor involved in signal transduction.
Purpose of the Study:
- To investigate the distinct roles of CD22 tyrosine residues in signaling.
- To elucidate the recruitment mechanisms of SHP-1 and Grb2 to CD22.
- To understand how CD22 regulates B cell signaling pathways.
Main Methods:
- BCR ligation in human and murine B cells.
- Tyrosine phosphorylation analysis of CD22.
- Assessment of SHP-1 and Grb2 recruitment to CD22.
- Inhibition studies using PP1 (Src family kinase inhibitor).
Main Results:
- CD22 is rapidly tyrosyl-phosphorylated upon BCR ligation.
- Two distinct tyrosine residues in CD22 are crucial for SHP-1 and Grb2 recruitment.
- SHP-1 recruitment is dependent on ITIMs, while Grb2 recruitment is independent.
- Lyn and Syk kinases are essential for CD22 phosphorylation.
Conclusions:
- CD22 possesses distinct signaling domains regulating BCR-initiated pathways.
- Differential recruitment of SHP-1 and Grb2 to CD22 influences B cell fate.
- Understanding CD22 phosphorylation is key to B cell signaling modulation.
Abstract:
Recognition of antigen by the B cell antigen receptor (BCR) determines the subsequent fate of a B cell and is regulated in part by the involvement of other surface molecules, termed coreceptors. CD22 is a B cell-restricted coreceptor that gets rapidly tyrosyl-phosphorylated and recruits various signaling molecules to the membrane following BCR ligation. Although CD22 contains three immunoreceptor tyrosine-based inhibitory motifs (ITIMs), only the two carboxyl-terminal ITIM tyrosines are required for efficient recruitment of the SHP-1 phosphatase after BCR ligation. Furthermore, Grb2 is inducibly recruited to CD22 in human and murine B cells. Unlike SHP-1, Grb2 recruitment to CD22 is not inhibited by specific doses of the Src family kinase-specific inhibitor PP1. The tyrosine residue in CD22 required for Grb2 recruitment (Tyr-828) is distinct and independent from the two ITIM tyrosines required for efficient SHP-1 recruitment (Tyr-843 and Tyr-863). Individually both Lyn and Syk are required for maximal phosphorylation of CD22 following ligation of the BCR, and together Lyn and Syk are required for all of the constitutive and induced tyrosine phosphorylation of CD22. We propose that the cytoplasmic tail of CD22 contains two domains that regulate signal transduction pathways initiated by the BCR and B cell fate.
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