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Membrane trafficking of CD1c on activated T cells

M del C Salamone1, A K Mendiguren, G V Salamone

  • 1Immunogenetics Division, University Hospital, School of Medicine, University of Buenos Aires, Argentina. marys@sinectis.com.ar

Journal of Leukocyte Biology
|October 9, 2001
PubMed

Insights

CD1c antigen expression on T cells is regulated by cell activation and intracellular trafficking. Membrane expression is temperature-dependent, with CD1c cycling between cell surface and intracellular compartments.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD1c antigen plays a role in T cell activation.
  • Understanding CD1c regulation and trafficking is crucial for immune response studies.

Purpose of the Study:

  • To investigate the regulation and intracellular trafficking of CD1c antigen on activated T cells.
  • To determine the factors influencing CD1c membrane expression.

Main Methods:

  • Flow cytometry
  • Reverse transcriptase-PCR (RT-PCR)
  • Immunocytochemical staining
  • Monoclonal antibody (mAb) binding assays at different temperatures

Main Results:

  • Phytohemagglutinin (PHA) activation induces CD1c transcripts for soluble, membrane, and cytoplasmic isoforms.
  • CD1c shows both cell membrane and cytoplasmic/perinuclear localization.
  • Membrane expression of CD1c is undetectable at 4°C and 37°C but detectable at room temperature.
  • Activated cells accumulate anti-CD1c mAbs intracellularly at physiological temperatures, indicating cycling.
  • CD1c exocytosis is sensitive to Brefeldin A, cytochalasin B, and chloroquine.

Conclusions:

  • CD1c membrane expression on activated T cells is tightly regulated and temperature-dependent.
  • CD1c undergoes intracellular trafficking and cycling between cellular compartments.
  • The exocytosis pathway of CD1c is sensitive to specific inhibitors, suggesting active transport mechanisms.

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