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[Evaluation of immunochromatography method for rapid detection of influenza A and B viruses]
C Kawakami1, H Shimizu, S Watanabe
1Yokohama City Institute of Health.
Insights
The QuickVue Influenza test accurately detects influenza A and B viruses, showing high specificity in clinical evaluations. This rapid diagnostic tool is suitable for identifying patients needing antiviral therapy.
Area of Science:
- Virology
- Immunochromatography
- Diagnostic Assays
Context:
- Influenza viruses pose a significant public health threat, necessitating rapid and accurate diagnostic methods.
- Existing diagnostic kits vary in sensitivity, specificity, and turnaround time.
- The QuickVue Influenza test utilizes immunochromatography for rapid viral detection.
Purpose:
- To evaluate the performance of the QuickVue Influenza test for detecting influenza A and B viruses.
- To determine the reactivity, detection limits, sensitivity, and specificity of the QuickVue Influenza test compared to cell culture and RT-PCR.
Summary:
- The QuickVue Influenza test demonstrated positive results for all twelve tested influenza strains and negative results for fourteen other respiratory viruses.
- Clinical evaluation using 92 throat swab specimens showed a sensitivity of 75.5% and specificity of 93.0% compared to cell culture.
- Compared to RT-PCR, the test exhibited a sensitivity of 72.2% and a specificity of 97.4%, with a low false positive rate.
Impact:
- The QuickVue Influenza test is a simple, one-step diagnostic tool with performance comparable to conventional methods.
- Its high specificity and rapid results make it suitable for diagnosing influenza and identifying patients requiring prompt antiviral treatment.
- This assay can aid in timely clinical decision-making and management of influenza outbreaks.
Abstract:
We have evaluated a new rapid detection kit for influenza A and B viruses, known as the QuickVue Influenza test (Quidel Coporation, USA); which is based on immunochromatography using virus isolates and clinical specimens. Twelve strains of influenza A and B were tested for evaluate the reactivity and detection limits of this test. The QuickVue Influenza test showed a positive result for all twelve strains of influenza virus and a negative result for fourteen different kinds of other respiratory viruses. The detection limits for six strains were 5 to 30 pfu/ml for a cell culture, 1.0 x 10(3) to 6.0 x 10(4) pfu/ml for 1st PCR, 1 to 50 pfu/ml for nested PCR, 3.0 x 10(5) to 6.0 x 10(5) pfu/ml for the QuickVue Influenza test, 1.5 x 10(5) to 1.0 x 10(6) pfu/ml for the Directigen Flu A, and 7.5 x 10(5) to 5.0 x 10(6) pfu/ml for the FLU OIA. Furthermore, the QuickVue Influenza test were clinically evaluated using 92 throat swab specimens collected from patients with influenza-like illnesses. By cell culture, influenza viruses were detected in 49 of the 92 specimens (AH1N1: 20, AH3N2: 7, B: 22); the titers of the influenza viruses were between 2.5 pfu/ml and 7.0 x 10(5) pfu/ml. Compared to cell culture, the QuickVue Influenza test showed a sensitivity of 75.5%, a specificity of 93.0%, a positive predictive value of 92.5%, a negative predictive value of 76.9%, and an efficiency value of 83.7%. On the other hand, influenza viruses were detected in 54 of the 92 specimens (AH1N1: 19, AH 3N2: 10, B: 25) by RT-PCR. Compared to RT-PCR, the QuickVue Influenza test showed a sensitivity of 72.2%, a specificity of 97.4%, a positive predictive value of 97.5%, a negative predictive value of 71.2%, and an efficiency value of 82.6%. Overall, only one throat swab specimen produced a false positive result using the QuickVue Influenza test; thus, this test appears to have a high specificity. We conclude that the QuickVue Influenza test is a simple one-step test with a sensitivity and specificity equivalent to those of other conventional diagnostic kits. The test is useful and suitable for the diagnosis of influenza and for identifying influenza patients requiring antiviral therapy.
