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[Study on the immunologic function of lymphocyte in cord blood]
1Central Lab of Hematological Department, Anhui Provincial Hospital, Hefei 230001, China.
Insights
Human cord blood (HUCB) T cells have a higher proportion of naive cells and lower cytokine production, potentially explaining reduced GVHD in HUCB transplantation and supporting their use in immunotherapy.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Context:
- Human cord blood (HUCB) T lymphocytes are increasingly recognized for their therapeutic potential.
- Understanding their immunologic function is crucial for optimizing clinical applications.
- Adult peripheral blood (PB) T lymphocytes serve as a control for comparative analysis.
Purpose:
- To investigate the immunologic characteristics of T lymphocytes in human cord blood.
- To establish a theoretical foundation for the clinical utilization of HUCB.
- To compare T cell function between HUCB and adult peripheral blood.
Summary:
- HUCB T cells exhibit a higher CD4+/CD8+ ratio and a greater percentage of naive CD45RA+ T cells compared to adult PB.
- HUCB T cells demonstrate lower production of interferon-gamma (IFN-γ) and interleukin-4 (IL-4) upon stimulation.
- While responsive to PHA stimulation, HUCB T cell activation kinetics differ from adult PB T cells.
Impact:
- The reduced cytokine secretion and high naive T cell percentage in HUCB may contribute to a lower incidence of graft-versus-host disease (GVHD).
- These findings offer a potential explanation for successful HUCB transplantation outcomes.
- HUCB T cells represent a promising source for adoptive immunotherapy strategies.
Objective:
To explore immunologic function of T lymphocytes in human cord blood (HUCB) and provide theoretical basis for clinical application of HUCB.
Methods:
Immunophenotype and co-stimulated molecules were detected by flow-cytometry using a panel of double labelled McAb, intracellular cytokines (interferon-gamma, interleukin-4) secreted by T lymphocytes by micro-culture of whole blood, and CD25 expression of T lymphocyte by PHA stimulation. T lymphocytes from adult peripheral blood (PB) were used as control.
Results:
There are a higher CD4+/CD8+ ratio of T subset and a lower percentage of CD8+ lymphocytes in HUCB than that in adult PB (P < 0.01), and more than 90% of HUCB T-cells co-expressed CD45RA naive antigen which was significant higher than the percentage in adult PB (P < 0.01). The percentages of IFN-gamma and IL-4-producing CD4+ and CD8+ T cells from HUCB were lower than that of the control group. T cells in HUCB were able to be activated by PHA, but the phases of activation differed from adult PB T cells.
Conclusions:
It suggested that the low ability to secret cytokines and high percentage of naive T cells might be responsible for the decreased incidence of acute and chronic GVHD and provided possible explanation for the clinical result in HUCB transplantation. T cells in HUCB is a potential resource of adoptive immunotherapy.