Related Experiment Video

Updated: Aug 15, 2026

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
12:58

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

Published on: May 10, 2017

[Production of cytokines from blood donor leukocytes]

O N Shcheglovitova, O V Parshina, T S Guseva

    Voprosy Virusologii
    |January 12, 2002
    PubMed

    Insights

    Donor leukocytes produce cytokines like IL-1 beta, IL-6, and TNF-alpha upon LPS stimulation. Inductor type and dose control cytokine levels, enabling modulation of immune responses.

    Area of Science:

    • Immunology
    • Molecular Biology

    Context:

    • Investigating in vitro cytokine production from donor blood leukocytes.
    • Assessing the impact of lipopolysaccharide (LPS) stimulation on immune cells.

    Purpose:

    • To determine the conditions under which LPS induces cytokine production (IL-1 beta, IL-6, TNF-alpha).
    • To explore the dose-dependent and inductor-specific effects on cytokine synthesis.
    • To evaluate the potential for modulating cytokine production.

    Summary:

    • LPS stimulation induced interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-alpha) production by donor leukocytes when spontaneous levels were low.
    • A statistically insignificant increase in cytokine production was observed when spontaneous production was already high.
    • Cytokine concentrations were dependent on the type and dosage of the inductors used.

    Impact:

    • The findings highlight the controllable nature of cytokine production via specific inductors.
    • This modulation capability could be leveraged for therapeutic strategies in immune-related conditions.
    • Provides insights into the nuanced regulation of inflammatory mediators by external stimuli.

    More Related Videos

    Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry12:36

    Single-cell Analysis of Immunophenotype and Cytokine Production in Peripheral Whole Blood via Mass Cytometry

    Published on: June 26, 2018

    Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment11:11

    Isolation and Expansion of Cytotoxic Cytokine-induced Killer T Cells for Cancer Treatment

    Published on: January 24, 2020

    Related Concept Videos

    Differentiation of Common Myeloid Progenitor Cells01:15

    Differentiation of Common Myeloid Progenitor Cells

    Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
    Regulation of Hematopoietic Stem Cells01:01

    Regulation of Hematopoietic Stem Cells

    All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
    Structure and Function of Leukocytes01:21

    Structure and Function of Leukocytes

    An adult in good health typically has between 4,500 and 11,000 leukocytes, or white blood cells, per microliter of blood, which constitutes about 1% of the total blood volume. Unlike red blood cells, white blood cells contain a nucleus and other cellular organelles but do not have hemoglobin. Most white blood cells reside in connective tissues, particularly in lymphatic organs such as the lymph nodes, with only a small fraction present in circulating blood.
    White blood cells protect the body...