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Published on: May 2, 2013
Detection of thoracotomy-induced alterations in cell- and humoral-mediated immune response
Gulbu Isitmangil1, Turgut Isitmangil, Kunter Balkanli
1Department of Immunology, School of Medicine, Yeditepe University, Istanbul, Turkey. gulbu@hotmail.com
Insights
Thoracotomy surgery causes an early increase in white blood cells (leukocytes) and a decrease in lymphocytes. However, operative stress does not significantly alter lymphocyte surface markers or immunoglobulin levels.
Area of Science:
- Immunology
- Surgical Complications
- Cellular Immunity
Background:
- Thoracotomy is a surgical procedure with known complications.
- Understanding the impact of thoracotomy on the immune system is crucial for patient recovery.
Purpose of the Study:
- To investigate the effects of thoracotomy on cellular and humoral immunity.
- To analyze changes in leukocyte and lymphocyte counts, surface markers, and immunoglobulin levels post-surgery.
Main Methods:
- Leukocyte and lymphocyte counts were determined preoperatively and at multiple time points postoperatively.
- Lymphocyte surface markers (CD3, CD4, CD8, CD19, CD16/56) and immunoglobulin levels (IgG, IgA, IgM, IgE) were measured using flow cytometry and ELISA.
Main Results:
- A significant increase in leukocyte count and a significant decrease in lymphocyte count were observed post-thoracotomy (P<0.001).
- No significant alterations were found in lymphocyte surface marker levels or immunoglobulin concentrations in the postoperative period (P>0.2).
Conclusions:
- Thoracotomy leads to early postoperative leucocytosis and lymphocytopenia.
- Operative stress from thoracotomy does not appear to affect immunoglobulin levels or lymphocyte subpopulations.
Objective:
It is well known that thoracotomy leads to several complications. In this study, effects of thoracotomy on cellular and humoral immunities have been investigated. Leukocyte counts and lymphocyte counts of 100 patients operated by thoracotomy have been determined preoperatively and on the postoperative 3rd hour, and 1st, 2nd, 3rd, 5th days. Also lymphocyte surface markers (CD3, CD4, CD8, CD4/CD8, CD19, CD16/56) and immunoglobulin levels (IgG, IgA, IgM, IgE) in 40 out of 100 patients in the preoperative period and postoperatively twice on 7th day and then in the 3rd week have been detected.
Materials And Methods:
For the methodology hemocounter, flow cytometer, immunoprecipitation, and enzyme-linked immunosorbent assay were used.
Results:
A marked increase in leukocyte count while a marked decrease in lymphocyte count has been observed after thoracotomy (P<0.001). There was not any significant alteration in levels of lymphocyte surface markers and immunoglobulins in the postoperative period (P>0.2).
Conclusion:
According to these results, leucocytosis occurred but lymphocyte count decreased in the early postoperative period. Immunoglobulin levels and subpopulation of lymphocytes were not affected from the operative stress.
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