Related Experiment Video

Updated: Aug 11, 2026

IP-FCM: Immunoprecipitation Detected by Flow Cytometry
12:17

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

Published on: December 2, 2010

An unusual Fc receptor-related protein expressed in human centroblasts

Fabio Facchetti1, Marina Cella, Silvana Festa

  • 1Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid, St. Louis, MO 63110, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 14, 2002
PubMed

Insights

Researchers identified Fc receptor homolog expressed in B cells (FREB), a novel B cell molecule. FREB is expressed in germinal center B cells and some B cell lymphomas, offering a potential marker for diffuse large B cell lymphoma.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Fc receptor homolog expressed in B cells (FREB) is a novel B cell-specific molecule.
  • FREB is distantly related to FcgammaRI and encoded on human chromosome 1q.
  • FREB exhibits intracellular distribution and lacks a transmembrane domain or immunoglobulin binding capacity.

Purpose of the Study:

  • To identify and characterize a novel B cell-specific molecule, FREB.
  • To investigate the expression patterns and potential functions of FREB in B cell differentiation and malignancies.
  • To evaluate FREB as a potential diagnostic marker for B cell lymphomas.

Main Methods:

  • Identification of FREB using molecular cloning techniques.
  • Analysis of FREB gene localization to human chromosome 1q.
  • Characterization of FREB protein distribution and immunoglobulin binding properties.
  • Immunohistochemical analysis using specific monoclonal antibodies to determine FREB expression in normal and malignant B cells.

Main Results:

  • FREB is a unique B cell-specific molecule, distantly related to FcgammaRI.
  • FREB is expressed intracellularly, lacks a transmembrane domain, and does not bind immunoglobulins.
  • FREB is preferentially expressed in germinal center centroblasts, key players in B cell maturation.
  • FREB expression is detected in a subset of diffuse large B cell lymphomas, originating from centroblasts.

Conclusions:

  • FREB possesses distinct characteristics suggesting a unique role in B cell differentiation.
  • FREB serves as a potential unique marker for the characterization of diffuse large B cell lymphomas.
  • Further research into FREB's function could elucidate novel B cell signaling pathways.

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 406:56

A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

Published on: March 10, 2018

Related Concept Videos

Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Enzyme-linked Receptors01:00

Enzyme-linked Receptors

Enzyme-linked receptors are proteins that act as both receptor and enzyme, activating multiple intracellular signals. This is a large group of receptors that include the receptor tyrosine kinase (RTK) family. Many growth factors and hormones bind to and activate the RTKs.
Neurotrophin (NT) receptors are a family of RTKs, including trkA, trkB, and trkC (tropomyosin-related kinase) receptors. TrkA is specific for nerve growth factor (NGF), neurotrophin-6, and neurotrophin-7. TrkB binds...
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
Introduction to Fibroblasts01:09

Introduction to Fibroblasts

Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
Receptor Tyrosine Kinases01:26

Receptor Tyrosine Kinases

Receptor tyrosine kinases or RTKs are membrane-bound receptors that phosphorylate specific tyrosine on protein substrates. RTKs regulate cellular growth, differentiation, survival, and migration. They contain an extracellular ligand binding domain, a transmembrane domain, and a cytosolic tail with intrinsic kinase activity. Several extracellular signaling molecules activate RTKs in one or more ways and relay the signal downstream. Ligands such as platelet-derived growth factor (PDGF) or...