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Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma
10:52

Tumor Engraftment in a Xenograft Mouse Model of Human Mantle Cell Lymphoma

Published on: March 30, 2018

CD5- mantle cell lymphoma

Zach Liu1, Henry Y Dong, Wojciech Gorczyca

  • 1IMPATH, New York, NY 10019, USA.

American Journal of Clinical Pathology
|August 7, 2002
PubMed

Insights

Mantle cell lymphoma (MCL) can lack CD5 expression, challenging diagnosis. Evaluating bcl-1 protein is crucial for identifying these CD5-negative MCL cases.

Area of Science:

  • Hematology
  • Oncology
  • Pathology

Background:

  • Mantle cell lymphoma (MCL) is typically characterized by B-cell antigen and CD5 expression, along with bcl-1 protein overexpression.
  • Unusual presentations of bcl-1 positive and CD5 negative MCL pose diagnostic and management difficulties.

Purpose of the Study:

  • To investigate cases of CD5-negative lymphoma with features consistent with MCL.
  • To determine the diagnostic utility of bcl-1 expression in the absence of CD5.

Main Methods:

  • Analysis of 25 cases (48 samples) of bcl-1 positive and CD5 negative lymphoma.
  • Assessment of CD5 expression using flow cytometry and immunohistochemistry.
  • Morphological evaluation for MCL features (centrocytic and blastic variants).
  • Cytogenetic analysis including fluorescence in situ hybridization (FISH) for t(11;14) translocation.

Main Results:

  • Twenty-five cases of bcl-1 positive, CD5 negative lymphoma were identified.
  • Morphological features consistent with MCL were observed in 20 cases (centrocytic) and 5 cases (blastic variant).
  • The characteristic t(11;14) translocation was confirmed in 8 of 11 cases by FISH and in 2 additional cases by cytogenetics.

Conclusions:

  • Mantle cell lymphoma can present without CD5 expression.
  • Immunohistochemical evaluation of bcl-1 or molecular genetic testing is essential for diagnosing MCL when CD5 is negative but clinical or morphological suspicion remains.

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