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Updated: Sep 29, 2026

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method
16:07

Determination of the Relative Potency of an Anti-TNF Monoclonal Antibody (mAb) by Neutralizing TNF Using an In Vitro Bioanalytical Method

Published on: September 16, 2017

Effect of cryoglobulin and crystalcryoglobulin on TNF-alpha production by normal human monocytes

Barbara Bucki1, Romuald Champy, Lucille Musset

  • 1Laboratoire de radiologie expérimentale et de physiopathologie articulaire, hĵpital Lariboisière, Assistance publique-hĵpitaux de Paris, AP-HP, France.

Joint Bone Spine
|August 20, 2002
PubMed

Insights

IgG1-kappa cryocrystalglobulin did not affect tumor necrosis factor-alpha (TNF-α) production in human monocytes. Noncrystallized cryoglobulins, however, did increase TNF-α levels, suggesting Fc region changes may alter cryocrystalglobulin activity.

Area of Science:

  • Immunology
  • Rheumatology
  • Molecular Biology

Background:

  • Destructive arthropathy can involve cryocrystalglobulin deposition in synovial fluid.
  • The proinflammatory role of IgG1-kappa cryocrystalglobulin in arthropathy is not well understood.

Purpose of the Study:

  • To investigate the proinflammatory properties of IgG1-kappa cryocrystalglobulin.
  • To determine its effect on tumor necrosis factor-alpha (TNF-α) production by human monocytes.

Main Methods:

  • Human monocytes were isolated and cultured.
  • Monocytes were exposed to cryocrystalglobulin, noncrystallized cryoglobulin, monosodium urate (MSU) crystals, or lipopolysaccharide (LPS).
  • Supernatant TNF-α concentrations were measured using ELISA.

Main Results:

  • IgG1-kappa cryocrystalglobulin did not stimulate TNF-α production by monocytes.
  • Noncrystallized cryoglobulin induced a time- and concentration-dependent increase in TNF-α.
  • The TNF-α increase from noncrystallized cryoglobulin was less than that induced by MSU crystals or LPS.

Conclusions:

  • IgG1-kappa cryocrystalglobulin lacks direct proinflammatory effects on human monocytes.
  • Alterations in the Fc region of the cryocrystalglobulin molecule may account for its lack of TNF-α induction.
Abstract

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