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B cells express Ly-6C in a Th1 but not Th2 cytokine environment

Annette J Schlueter1, Arthur M Krieg, Peter De Vries

  • 1Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242-1181, USA. schluetera@uihc.uiowa.edu

Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research
|August 20, 2002
PubMed

Insights

Pro-inflammatory Th1 cytokines, especially Interferon-alpha (IFN-alpha), transiently induce Ly-6C expression on B cells. Th2 cytokines do not induce Ly-6C, and Ly6.1 mice show greater expression than Ly6.2 strains.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • B cells typically do not express Ly-6C in a resting state.
  • Ly-6C expression on B cells has been observed following exposure to pro-inflammatory stimuli.
  • Understanding the regulation of Ly-6C expression on B cells is crucial for comprehending immune responses.

Purpose of the Study:

  • To investigate the factors controlling Ly-6C expression on B cells.
  • To determine the kinetics of Ly-6C expression in B cells under various conditions.
  • To compare Ly-6C expression regulation between different mouse strains (Ly6.1 vs. Ly6.2).

Main Methods:

  • In vivo studies using mouse models to assess cytokine effects on B cell Ly-6C expression.
  • In vitro experiments exposing B cells to various cytokines (IFN-alpha, IFN-gamma, IL-4) and activators (anti-IgM, CD40 ligand).
  • Comparative analysis of Ly-6C expression in Ly6.1 and Ly6.2 mouse strains.

Main Results:

  • Pro-inflammatory Th1 cytokines, particularly Interferon-alpha (IFN-alpha) and Interferon-gamma (IFN-gamma), transiently upregulate Ly-6C expression on B cells.
  • Polyclonal B cell activators showed limited independent induction but enhanced IFN-induced Ly-6C expression.
  • Th2 cytokine environments, including Interleukin-4 (IL-4), did not induce Ly-6C expression and IL-4 antagonized IFN-driven induction.
  • Ly6.1 mouse strains exhibited a greater capacity for B cell Ly-6C expression compared to Ly6.2 strains.

Conclusions:

  • Th1, but not Th2, cytokine environments can transiently induce Ly-6C expression on B cells.
  • IFN-alpha and IFN-gamma are key inducers of B cell Ly-6C expression.
  • Significant differences exist in Ly-6C expression regulation between Ly6.1 and Ly6.2 mouse strains.

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