Related Experiment Video
Updated: Aug 8, 2026
An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets
Published on: April 18, 2016
Involvement of LOX-1 in dendritic cell-mediated antigen cross-presentation
Yves Delneste1, Giovanni Magistrelli, Jean Gauchat
1Centre d'Immunologie Pierre Fabre, 5 avenue Napoléon III, Saint Julien en Genevois, France.
Insights
Scavenger receptors, particularly LOX-1, facilitate antigen uptake by dendritic cells for cross-priming. Targeting LOX-1 with tumor antigens can induce antitumor immunity, showing its promise in cancer immunotherapy.
Area of Science:
- Immunology
- Cell Biology
- Cancer Research
Background:
- Cross-priming initiates T-cell responses via MHC class I pathway.
- Dendritic cell receptors for antigen endocytosis in cross-priming are largely unidentified.
Purpose of the Study:
- Identify receptors responsible for heat shock protein (HSP) uptake by dendritic cells.
- Investigate the role of identified receptors in antigen cross-presentation and anti-tumor immunity.
Main Methods:
- Utilized human dendritic cells to study scavenger receptor binding of HSPs.
- Employed neutralizing anti-LOX-1 monoclonal antibodies (mAbs) to assess LOX-1 function.
- Evaluated in vivo anti-tumor immune responses following LOX-1 targeting.
Main Results:
- Scavenger receptors are the primary HSP-binding structures on human dendritic cells.
- LOX-1 was identified as a key scavenger receptor involved in HSP binding.
- Anti-LOX-1 mAb inhibited HSP70 binding and subsequent antigen cross-presentation.
- Targeting LOX-1 with tumor antigens in vivo successfully induced anti-tumor immunity.
Conclusions:
- LOX-1 is a crucial scavenger receptor for HSP uptake and antigen cross-presentation by dendritic cells.
- LOX-1 represents a promising therapeutic target for developing novel cancer immunotherapies.
Abstract:
Some exogenous antigens, such as heat shock proteins or apoptotic bodies, gain access to the MHC class I processing pathway and initiate CTL responses, a process called cross-priming. To be efficient in vivo, this process requires endocytosis of the antigen by dendritic cells via receptors which remain unidentified. Here, we report that scavenger receptors are the main HSP binding structures on human dendritic cells and identify LOX-1 as one of these molecules. A neutralizing anti-LOX-1 mAb inhibits Hsp70 binding to dendritic cells and Hsp70-induced antigen cross-presentation. In vivo, to target LOX-1 with a tumor antigen using an anti-LOX-1 mAb induces antitumor immunity. Thus, the scavenger receptor LOX-1 is certainly a promising target for cancer immunotherapy.
Related Concept Videos
01:18Lymphoid Cells and Tissues
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
01:26Antigens Involved in Adaptive Immunity
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
01:22Antigen Presenting Cells
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
01:31Antigen Processing Pathways
MHC Class I: Presenting Endogenous...
01:22T Cell Activation and Clonal Selection
Naive T cells that have not yet encountered an antigen express two primary CD...
01:24B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...