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Updated: Aug 9, 2026
Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
Published on: May 8, 2016
[Isolation and phenotypic analysis of lamina propria mononuclear cells from colonoscopic biopsy specimens]
1Department of Gastroenterology, The First Affiliated Hospital, WCUMS, Chengdu 610041.
Insights
This study successfully isolated lamina propria mononuclear cells (LPMC) from biopsy samples, finding higher yields in ulcerative colitis (UC) patients. This method is effective for mucosal immune research in gut disorders like UC.
Area of Science:
- Immunology
- Gastroenterology
Context:
- Investigating the immune cell composition of the gut mucosa is crucial for understanding inflammatory bowel diseases.
- Standardized methods for isolating and analyzing these cells from biopsy specimens are needed.
Purpose:
- To establish a reliable method for isolating lamina propria mononuclear cells (LPMC) from mucosal biopsy specimens.
- To perform phenotypic analysis of LPMC and compare cell yields and lymphocyte subsets between patients with ulcerative colitis (UC), colorectal cancer, and healthy controls.
Summary:
- Lamina propria mononuclear cells (LPMC) were isolated from biopsy specimens of UC patients, controls, and cancer patients.
- UC patients showed significantly higher LPMC yields and a distinct T-cell subset profile (increased CD4+ T cells, decreased CD8+ T cells) compared to controls.
- The isolation method provided sufficient cell yield and viability for phenotypic analysis.
Impact:
- The study demonstrates a robust method for LPMC isolation and analysis from biopsy samples.
- Findings highlight potential immune dysregulation in UC, characterized by altered T-cell populations.
- This technique can advance research into the mucosal immunology of gastrointestinal disorders.
Abstract:
This study was directed to the method of isolation of lamina propria mononuclear cells (LPMC) from mucosal biopsy specimens and the phenotypic analysis of the cells. Ten biopsy specimens, taken individually from 8 patients with ulcerative colitis (UC), 5 normal controls and 22 patients with colorectal cancer, were collected to compare the factors that influence isolation and cell yield and to analyze the lymphocyte subsets by means of two-color flow cytometry. LPMC yield averaged 10(6) with a viability of more than 95% in UC, twice the yields in other two groups. The percentages of total T, B cells in three groups were similar (P > 0.05). When the UC group was compared with the other two groups, a significantly higher proportion of CD3(+)-CD4(+)-T cell (49.98% vs 37.54% and 37.25%, P < 0.01) was noted, whereas a lower proportion of CD3(+)-CD8(+)-T cell (23.64% vs 31.52% and 31.07%, P < 0.01) was observed. These data showed that the yield and viability of LPMC isolated from biopsy specimens were good enough for a phenotypic or functional analysis, which might be helpful to mucosal immune research on gut disorders, such as UC.
