Related Experiment Video

Updated: Aug 1, 2026

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection
09:01

An In vitro Model to Study Immune Responses of Human Peripheral Blood Mononuclear Cells to Human Respiratory Syncytial Virus Infection

Published on: December 10, 2013

[Human herpesvirus-6 infection induces IL-10 production in monocytes]

C Li1, J M Goodrich, X Yang

  • 1Children's Hospital, Chongqing University of Medical Sciences, Chongqing 630014.

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology
|January 8, 2003
PubMed

Insights

Interleukin 10 (IL-10) inhibits pro-inflammatory cytokine production during human herpesvirus 6 (HHV-6) infection. This suggests IL-10 plays a role in HHV-6 immune pathogenesis and latent infection.

Area of Science:

  • Immunology
  • Virology
  • Molecular Biology

Context:

  • Human herpesvirus 6 (HHV-6) is a common viral infection with complex interactions with the host immune system.
  • Understanding the immune response to HHV-6 is crucial for elucidating its pathogenesis and potential for latency.
  • Cytokines play a critical role in modulating immune responses during viral infections.

Purpose:

  • To investigate the role of interleukin 10 (IL-10) and other cytokines in the immune pathogenesis of HHV-6 infection.
  • To explore the regulatory mechanisms of cytokine production in HHV-6 infected monocytes.
  • To determine the impact of endogenous IL-10 on pro-inflammatory cytokine expression during HHV-6 infection.

Summary:

  • Reverse transcription-polymerase chain reaction (RT-PCR) and sandwich ELISA were used to analyze cytokine expression in HHV-6 infected monocytes.
  • Results indicated that IL-10 expression increased with HHV-6 infection, while transcription of IL-1 beta, IL-6, and TNF-alpha mRNA decreased.
  • Blocking endogenous IL-10 led to a significant increase in TNF-alpha, IL-1 beta, and IL-6 production, demonstrating IL-10's inhibitory role at the transcriptional level.

Impact:

  • The findings suggest that endogenous IL-10 production contributes to the immune dysregulation observed in HHV-6 infection.
  • IL-10's ability to suppress Th1 responses and down-regulate monocyte/macrophage function may facilitate HHV-6 latency.
  • This study provides insights into the intricate interplay between HHV-6 and the host immune system, potentially informing therapeutic strategies.

More Related Videos

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis08:44

Separation of Immune Cell Subpopulations in Peripheral Blood Samples from Children with Infectious Mononucleosis

Published on: September 7, 2022

Related Concept Videos

T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
Immunodeficiency Diseases01:25

Immunodeficiency Diseases

Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
There are three main causes of immunodeficiency disorders...
Herpes01:28

Herpes

Herpes simplex type 1 (HSV‑1) is a widespread pathogen responsible for orolabial lesions. It is an enveloped, double-stranded DNA (dsDNA) virus belonging to the family Herpesviridae. Once the virus infects a host cell, its double‑stranded DNA genome is delivered into the nucleus, where a coordinated cascade of immediate‑early, early, and late gene expression directs viral DNA replication, structural protein synthesis, and virion assembly. After primary infection of epithelial cells, HSV-1...
Cytomegalovirus Disease01:27

Cytomegalovirus Disease

Cytomegalovirus (CMV) disease is caused by human cytomegalovirus, a double-stranded DNA virus of the Herpesviridae family. While primary CMV infection is often asymptomatic in immunocompetent individuals, the virus can cause severe disease in neonates and immunocompromised patients. CMV is the most common cause of congenital viral infection in the United States, and a major pathogen in solid organ and hematopoietic stem cell transplant recipients.CMV is transmitted via bodily fluids, sexual...
Genital Herpes01:23

Genital Herpes

Genital herpes is a sexually transmitted infection primarily caused by herpes simplex virus type 2 (HSV-2), though herpes simplex virus type 1 (HSV-1) is increasingly implicated in genital infections, particularly among younger populations. Transmission occurs mainly through sexual contact, with asymptomatic viral shedding serving as a major route of spread. This characteristic makes HSV-2 difficult to control at a population level, as individuals may unknowingly transmit the virus even in the...