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BCL-1 rearrangement in acute lymphocytic leukemia and its clinical significance
1Institute of Hematology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022.
Insights
BCL-1 rearrangement, a genetic alteration, was found in some cases of acute B lymphocytic leukemia (B-ALL). This rearrangement, along with cyclin D1 protein expression, indicates a poor prognosis for B-ALL patients.
Area of Science:
- Hematology
- Molecular Biology
- Oncology
Background:
- Acute lymphocytic leukemia (ALL) is a significant hematologic malignancy.
- The clinical significance of BCL-1 rearrangement and cyclin D1 protein expression in ALL requires further investigation.
Purpose of the Study:
- To investigate BCL-1 rearrangement (BCL-1/IgH gene rearrangement) in acute lymphocytic leukemia.
- To determine the clinical significance of BCL-1 rearrangement and cyclin D1 protein expression in ALL patients.
Main Methods:
- Genomic DNA from mononuclear cells of 38 ALL patients was analyzed using hemi-nested polymerase chain reaction (PCR).
- Cyclin D1 protein expression was assessed using immunohistochemical methods.
- Control groups included patients with granulocytic leukemia and normal bone marrow.
Main Results:
- BCL-1 rearrangement was detected in 7.9% (3/38) of ALL patients.
- Cyclin D1 protein expression was observed in 10.5% (4/38) of ALL patients.
- All three patients with BCL-1 rearrangement were diagnosed with B-cell ALL (B-ALL) and exhibited cyclin D1 protein expression, with significantly increased leukocyte counts and poor response to chemotherapy.
Conclusions:
- BCL-1/IgH gene rearrangement is detectable in acute B lymphocytic leukemia.
- B-ALL patients with BCL-1 rearrangement and/or cyclin D1 protein expression demonstrate a poor prognosis.
Abstract:
BCL-1 rearrangement (BCL-1/IgH gene rearrangement) in acute lymphocytic leukemia and its clinical significance was investigated. In 38 patients with acute lymphocytic leukemia (ALL), the genomic DNA of mononuclear cells isolated from peripheral blood and bone marrow was amplified by using hemi-nested polymerase chain reaction (PCR) technique and the expression of cyclin D1 protein of mononuclear cells was detected by using immunohistochemical method. Ten patients with acute granulocytic leukemia, 2 with chronic granulocytic leukemia and 10 with normal bone marrow served as control group. The results showed that BCL-1 rearrangement was detectable in 3 of 38 ALL patients (7.9%) and cyclin D1 protein positive expression was detected in 4 ALL patients (10.5%). Three ALL patients with BCL-1 rearrangement were all B-cell leukemia (B-ALL) and accompanied by cyclin D1 protein expression. No BCL-1/IgH rearrangement or cyclin D1 protein expression was detected in 12 patients with granulocytic leukemia and 10 cases of normal bone marrow. Leukocyte counts in peripheral blood of B-ALL patients with BCL-1 rearrangement and (or) cyclin D1 protein expression were significantly increased and the patients had bad reaction to chemotherapy. It was concluded that: 1) BCL-1/IgH gene rearrangement were detected in acute B lymphocytic leukemia; 2) B-ALL patients with BCL-1 rearrangement and (or) cyclin D1 protein expression had poor prognosis.
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