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Published on: November 14, 2016
Immunohistochemical observations on cellular response in unilocular hydatid lesions and lymph nodes of cattle
Tsukasa Sakamoto1, Perla A Cabrera
1Departamento de Parasitologia, Facultad de Veterinaria, Universidad de Republica, Montevideo, Uruguay. sakamoto@cvc-ohno.or.jp
Insights
Immunohistochemistry reveals distinct immune cell profiles in bovine hydatid lesions. Progressive cysts show CD8+ T cells, while regressive cysts feature CD4+ T cells and eosinophils, suggesting lymph node origins.
Area of Science:
- Veterinary immunology
- Parasitology
- Immunohistochemistry
Background:
- Hydatidosis is a significant zoonotic disease caused by Echinococcus granulosus.
- The immune response within hydatid lesions and draining lymph nodes in cattle remains poorly understood.
Purpose of the Study:
- To investigate the immunohistochemical characteristics of hydatid lesions and associated lymph nodes in cattle.
- To elucidate the role of different immune cell subpopulations in the pathogenesis of hydatid cysts.
Main Methods:
- Collection of hydatid cysts from cattle livers and lungs in Uruguay.
- Immunohistochemical staining of frozen tissue sections using monoclonal antibodies against CD markers (CD2, CD4, CD5, CD8), B cell markers, granulocyte-monocyte/macrophage markers, and bovine eosinophil-specific antiserum.
- Analysis of immune cell infiltration in the adventitial layer of hydatid cysts and local lymph nodes.
Main Results:
- Epithelioid cells in the adventitial layer were identified as macrophages.
- Progressive hydatid cysts showed a predominance of CD8+ T cells in the pericystic adventitia, with fewer CD4+ T cells.
- Regressive and involuted cysts exhibited infiltration primarily by CD4+ T cells and evidence of eosinophil-mediated destruction of the laminated layer.
- Immune cell subpopulations in draining lymph nodes mirrored those found in the adventitial layer of hydatid lesions.
Conclusions:
- The immune cell infiltrates in bovine hydatid lesions, including eosinophils and T cell subpopulations, likely originate from the draining lymph nodes.
- Distinct immune responses are associated with different stages of hydatid cyst development in cattle.
Abstract:
As we believe the immunohistochemistry of the hydatid lesions and draining lymph nodes has never been studied, we collected them from the liver and lungs of cattle in Uruguay for such a study. Frozen sections of the tissues were immunohistochemically stained using monoclonal antibodies against surface markers CD2, CD4, CD5, CD8, B cell and granulocyte-monocyte/macrophage and antiserum against specific granules of bovine eosinophils. The adventitial layer of the cyst wall consists of a layer of epithelioid cells and connective tissue. The cells from the epithelioid cell layer were a kind of macrophage. In most cases having progressive hydatid cysts, CD8+ cells were predominant in the pericystic adventitia, and a relatively small number of CD4+ cells were in the same area. In the adventitial layer surrounding the regressive and involutional hydatid cysts, infiltrating lymphocytes were composed mostly of CD4+ cells. An eosinophil-mediated destruction of the laminated layer was recognized in the regressive and involuted hydatid cysts. The subpopulations of T cells in the local lymph nodes tended to be similar to T cells in the adventitial layer of hydatid lesions. From our findings, we consider that infiltration of eosinophils and the subpopulations of lymphocytes infiltrating the hydatid lesions in the liver and lungs are derived from cells in the draining lymph nodes of both organs.
