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Surface CD4 expression modulated by a cellular factor induced by HIV type 1 infection

Dennis Sheeter1, Pinyi Du, Steffney Rought

  • 1Department of Medicine, University of California San Diego, La Jolla, California 92093-0679, USA.

AIDS Research and Human Retroviruses
|March 18, 2003
PubMed

Insights

Human immunodeficiency virus type 1 (HIV-1) infection alters host gene expression. This study found that nuclear factor I-B2 (NFI-B2) impairs CD4 transcription, reducing HIV-1 production.

Area of Science:

  • Molecular Biology
  • Virology
  • Immunology

Background:

  • Human immunodeficiency virus type 1 (HIV-1) infection leads to cellular dysfunction by altering host gene expression.
  • Understanding host-pathogen interactions is crucial for developing therapeutic strategies against HIV-1.

Purpose of the Study:

  • To investigate the role of host cell gene modulation during HIV-1 infection.
  • To elucidate the specific function of nuclear factor I-B2 (NFI-B2) in HIV-1 pathogenesis.

Main Methods:

  • Utilized high-density oligonucleotide microarray technology to identify modulated host genes in HIV-1 infected cells.
  • Generated a Jurkat T cell line with constitutive NFI-B2 expression for functional studies.
  • Analyzed viral production, surface CD4 expression, and CD4 mRNA levels.

Main Results:

  • HIV-1 infection modulated several host cell genes, including NFI-B2.
  • Constitutive NFI-B2 expression in Jurkat T cells reduced HIV-1 production.
  • NFI-B2 expression led to downregulation of surface CD4 and CD4 mRNA, indicating impaired CD4 transcription.
  • CXCR4 coreceptor expression remained unchanged.

Conclusions:

  • NFI-B2 plays a significant role in modulating CD4 expression during HIV-1 infection.
  • HIV-1-induced modulation of NFI-B2 may contribute to the reduction of cell surface CD4 expression.
  • NFI-B2 represents a potential target for therapeutic intervention in HIV-1 infection.

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