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Cytoplasmic expression of a CD24-related epitope in human PHA activated normal T lymphocytes

M C Salamone1, B Achino, L Fainboim

  • 1Laboratorio de Inmunogenética, Hospital de Clínicas José de San Martin, Buenos Aires, Argentina.

Immunology Letters
|October 1, 1992
PubMed

Insights

Researchers found that the CD24 antigen is expressed intracellularly on activated normal human T lymphocytes, appearing 24-48 hours after stimulation. This study provides the first evidence of CD24 expression in activated T cells.

Area of Science:

  • Immunology
  • Cell Biology
  • Hematology

Background:

  • CD24 is a cell surface antigen with known roles in B cell development and activation.
  • Its expression and function on normal human T lymphocytes remain less understood.

Purpose of the Study:

  • To investigate the expression and localization of the CD24 antigen on resting and activated normal human T lymphocytes.
  • To characterize the kinetics of CD24 expression following T cell activation.

Main Methods:

  • Immunoperoxidase (IPx) and indirect immunofluorescence (IIF) assays were employed.
  • Analysis was performed on resting and phytohemagglutinin (PHA)-activated normal human mononuclear cells, including T lymphocytes.
  • VIB-E3 mAb, an anti-CD24 antibody, was used for detection.

Main Results:

  • VIB-E3 mAb showed no reactivity with resting normal T lymphocytes.
  • Intracytoplasmic expression of CD24 was detected in PHA-activated normal mononuclear cells.
  • CD24 expression was observed to appear between 24 to 48 hours after PHA stimulation.

Conclusions:

  • This study presents the first evidence of CD24-related epitope expression in normal activated human T lymphocytes.
  • CD24 is expressed intracellularly on activated T cells, suggesting a potential role in T cell activation or function.

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