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Published on: March 13, 2013
Analysis of expression and soluble form of intercellular adhesion molecule-1 in malignant melanoma
T Kageshita1, A Yoshii, T Kimura
1Department of Dermatology, Kumamoto University School of Medicine, Japan.
Insights
Interferon (IFN) boosts Intercellular Adhesion Molecule-1 (ICAM-1) expression in melanoma cells, with IFN-gamma showing the strongest effect. Soluble ICAM-1 levels correlate with melanoma stage, suggesting its prognostic value.
Area of Science:
- Immunology
- Dermatology
- Cell Biology
Background:
- Intercellular Adhesion Molecule-1 (ICAM-1) is implicated in immune responses.
- The role of interferons (IFNs) in modulating ICAM-1 expression in melanoma is not fully understood.
Purpose of the Study:
- To investigate the effects of different types of IFNs on ICAM-1 expression and shedding in human melanoma cell lines.
- To correlate ICAM-1 expression in melanoma lesions and circulation with clinical parameters and prognosis.
Main Methods:
- Indirect binding assay and double determinant immunoassay (DDIA) were used to measure ICAM-1 expression and shedding.
- Immunostaining was performed on surgically removed melanoma lesions.
- Circulating ICAM-1 levels were measured in patients with different stages of melanoma.
Main Results:
- IFNs increased ICAM-1 expression on melanoma cell lines, with IFN-gamma being the most potent.
- ICAM-1 shedding into spent media correlated with cell surface expression.
- ICAM-1 expression in primary lesions correlated positively with lesion thickness and inversely with disease-free interval.
- Circulating ICAM-1 levels were significantly higher in stage 4 melanoma patients compared to stages 1-3.
Conclusions:
- Interferons modulate ICAM-1 expression in melanoma cells.
- Soluble ICAM-1 may play a role in host immunity against melanoma.
- ICAM-1 levels are a potential biomarker for evaluating melanoma prognosis.
Abstract:
The effects of interferon (IFN) on the expression of ICAM-1 in human melanoma cell lines and the shedding of ICAM-1 into spent media were investigated using an indirect binding assay and a double determinant immunoassay (DDIA). While IFN increased the level of expression of ICAM-1 on melanoma cell lines, the susceptibility to IFN differed according to cell line. The enhancing effect of IFN-gamma was concentration-dependent, and it exceeded those of IFN-alpha and IFN-beta. Shedding of ICAM-1 into spent media was detected effectively, and the results agreed well with the expression of ICAM-1 on melanoma cells. Immunostaining of the surgically removed melanoma lesions was positively correlated with the thickness of the primary lesion. The expression of ICAM-1 in primary melanoma lesions was inversely correlated with the disease-free interval. The circulating ICAM-1 with stage 4 melanoma patients significantly exceeded that of stage 1-3 melanoma patients. These observations suggest that ICAM-1 in its soluble form may play an important role in host immunities and may be useful in evaluating the prognosis of patients with melanoma.
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