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Updated: Aug 9, 2026
Generation of Human CD40-activated B cells
Published on: October 17, 2009
[Cloning of human B lymphocyte activation-related novel gene]
1National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS, PUMC, Beijing 100005, China. luxingwu@263.net
Insights
Researchers identified a novel gene linked to B lymphocyte activation using differential display and PCR. This discovery advances understanding of B cell biology and immune responses.
Area of Science:
- Molecular Biology
- Immunology
- Genetics
Context:
- B lymphocytes play a crucial role in adaptive immunity.
- Understanding B lymphocyte activation is key to immune system research.
- Gene expression profiling reveals cellular state changes.
Purpose:
- To identify and clone novel genes involved in human B lymphocyte activation.
- To characterize the genetic basis of B cell response.
Summary:
- Differential display-reverse transcription PCR (DDRT-PCR) identified unique mRNA transcripts between resting and activated human B lymphocytes.
- A novel cDNA clone of 2,048 bp, containing a 630 bp open reading frame, was isolated.
- The deduced amino acid sequence showed homology to the KAR3 protein, a kinesin superfamily member.
Impact:
- This research provides a new candidate gene potentially regulating B lymphocyte activation.
- The findings contribute to the molecular understanding of immune cell signaling.
- The identified gene may serve as a target for future therapeutic strategies in immune-related diseases.
Objective:
To clone the novel activation-related gene of B lymphocyte.
Methods:
The differential display reversal transcription PCR (DDRT-PCR) technique was applied to analyse the expression difference of mRNA between resting and activated B lymphocyte from human tonsil. The positive differential display cDNA fragment identified by Northern-blotting was chosen as probe to filtrate human activated B lymphocyte cDNA library.
Results:
Sixty two differential display cDNA fragments (expressed sequence tag, EST) were obtained. Thirty-two of them were mainly expressed in resting B lymphocyte and thirty were expressed in activated cells. Twenty-five were positive ones after identification by Northern blot analysis. A novel cDNA clone was obtained after using EST30 as a probe to filtrate the human activated B cell cDNA library. The whole cDNA clone was 2,048 bp in length and contains a 630 bp open reading frame. The N end of the deduced amino acid sequence was homologous with KAR3 protein which is a member of kinesins superfamily in yeast.
Conclusions:
A novel possible activation-related gene in human B lymphocyte was obtained.
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