Related Experiment Video

Updated: Aug 9, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Interleukin-1 induces c-fos and c-jun gene expression in T helper type II cells through different signal transmission

E Muñoz1, A M Zubiaga, B T Huber

  • 1Departamento de Bioquímica y Biología Molecular, Facultad de Medicina de Córdoba, Spain.

European Journal of Immunology
|August 1, 1992
PubMed

Insights

Interleukin-1 (IL-1) activates distinct signaling pathways in D10A cells. These pathways differentially regulate protooncogene expression, involving protein tyrosine kinase and protein kinase C.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Signaling

Background:

  • Interleukin-1 (IL-1) is a key cytokine involved in immune responses and cellular processes.
  • Protooncogene expression, such as c-jun and c-fos, is crucial for cell proliferation and differentiation.
  • Understanding IL-1 signaling pathways is vital for comprehending cellular regulation.

Purpose of the Study:

  • To elucidate the signal transduction pathways activated by IL-1 in the T helper 2 cell line D10A.
  • To investigate the mechanisms underlying IL-1-induced expression of c-jun and c-fos protooncogenes.

Main Methods:

  • Utilized the D10A T helper 2 cell line.
  • Investigated IL-1 signaling pathways using protein tyrosine kinase inhibitors (tyrphostin) and protein kinase C activators (phorbol esters).
  • Analyzed gene transcription and mRNA expression of c-jun and c-fos.
  • Examined the role of different Interleukin-1 Receptors (IL-1R) and intracellular cAMP levels.

Main Results:

  • IL-1 induced c-jun transcription and mRNA expression via a protein tyrosine kinase-dependent pathway linked to the 80-kDa IL-1R, independent of protein kinase C.
  • IL-1-mediated c-fos mRNA expression was dependent on protein kinase C and linked to a distinct, unidentified IL-1R.
  • Intracellular cAMP accumulation, generated through the 80-kDa IL-1R, negatively regulated IL-1-induced c-fos expression mediated by protein kinase C.

Conclusions:

  • IL-1 modulates c-fos expression in D10A cells through two independent IL-1Rs.
  • These distinct IL-1Rs are coupled to different signal transduction pathways, highlighting complex IL-1 regulatory mechanisms.

More Related Videos

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo05:44

The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo

Published on: April 25, 2016

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity06:16

Generation of Orthotopic Pancreatic Tumors and Ex vivo Characterization of Tumor-Infiltrating T Cell Cytotoxicity

Published on: December 8, 2019

Related Concept Videos

Intracellular Signaling Cascades01:24

Intracellular Signaling Cascades

Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...