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Aberrant expression of blood group H-antigen in Shope carcinoma cells
M Kyogashima1, M Inoue, J Nakayama
1Department of Microbiology, Shiga University of Medical Science, Otsu, Japan.
Insights
Blood group H-antigen, a glycolipid, was found in Shope carcinoma cells but not normal rabbit skin cells. This suggests aberrant synthesis in cancer cells, offering potential targets for therapy.
Area of Science:
- Oncology
- Glycobiology
- Immunology
Background:
- Blood group H-antigen is a key human blood group antigen.
- Aberrant antigen expression is common in cancer cells.
- Shope papilloma virus causes skin tumors in rabbits.
Purpose of the Study:
- To investigate the presence and role of blood group H-antigen in Shope carcinoma.
- To determine if H-antigen is aberrantly expressed in these cancer cells.
Main Methods:
- Thin-layer chromatography immunostaining
- Immunohistochemical analysis using anti-H monoclonal antibody
- Cytofluorometric analysis
- In vitro antibody-dependent cell lysis assays
Main Results:
- H-antigen was identified as glycolipids in Shope carcinoma cell lines.
- H-antigen was absent in normal epidermal and papilloma cells but present in hair follicle sheath cells.
- Shope carcinoma cells expressing H-antigen were lysed by antibody and complement.
Conclusions:
- H-antigen is aberrantly synthesized in Shope carcinoma cells.
- The presence of H-antigen on cancer cells may contribute to their susceptibility to immune attack.
Abstract:
Blood group H-antigen was clearly demonstrated as glycolipids in Shope carcinoma cells and its culture cell lines by thin-layer chromatography immunostaining and immunohistochemical analyses using the anti-H monoclonal antibody. This antigen was not detected in normal epidermal and Shope papilloma cells of rabbits but was present in the outer sheath cells of hair follicles of normal rabbit skin. The H-antigen was also detected by cytofluorometric analysis on Shope carcinoma cell lines and the cells were lysed in vitro by the antibody and complement in a dose-dependent manner. These results suggested that the H-antigen was aberrantly synthesized in epidermal carcinoma cells.