[Flow cytometric measurement of cell surface and intracellular antigens present in leukemia cells]
1Department of Pediatrics, Aichi Medical University.
Insights
Multiparameter flow cytometry enables detailed analysis of leukemia cells. Establishing standardized procedures is crucial for reliable clinical diagnostics and understanding cell biology.
Area of Science:
- Immunology
- Cell Biology
- Biotechnology
Context:
- Flow cytometry advancements allow multiparameter analysis of cellular antigens.
- Clinical use necessitates standardized protocols for sample handling and analysis.
- Characterizing human leukemia requires robust flow cytometric techniques.
Purpose:
- To outline essential procedures for multiparameter flow cytometry in clinical settings.
- To demonstrate the application of these techniques in human leukemia cell characterization.
- To highlight the diagnostic and biological insights gained from flow cytometry.
Summary:
- This article details the fundamental elements for establishing multiparameter flow cytometry procedures.
- It explores the application of these methods for characterizing human leukemia cells.
- Flow cytometric analysis of cell surface and intracellular antigens provides key diagnostic and biological information.
Impact:
- Facilitates the clinical application of advanced flow cytometry.
- Enhances the diagnostic accuracy for human leukemia.
- Improves understanding of leukemia cell biology through antigen profiling.
Abstract:
Recent advances in flow cytometric technology has enabled us to perform multiparameter analysis of cell surface and intracellular antigens. The clinical application of such analyses requires to establish the procedures of sample preparation, fixation, staining, instrument calibration and data analysis. This article describes the basic elements involved in establishing such procedures and explores the use of multiparameter flow cytometric analysis in the characterization of human leukemia cells. Flow cytometric measurements of cell surface and intracellular antigens of leukemia cells can provide important insights into the biologic features of these cells as well as significant diagnostic information.
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