Circulating ICAM-1 levels in serum of uveitis patients
E Arocker-Mettinger1, L Steurer-Georgiew, M Steurer
12nd Department of Ophthalmology, University of Vienna, Austria.
Insights
Circulating serum Intercellular Adhesion Molecule-1 (ICAM-1) levels were elevated in specific uveitis types, including HLA-B27 negative anterior uveitis and intermediate uveitis, as well as in sarcoidosis patients. Other uveitis forms showed no significant difference from controls.
Area of Science:
- Immunology
- Ophthalmology
- Inflammation Research
Background:
- Intercellular Adhesion Molecule-1 (ICAM-1) is a key inflammatory marker.
- A circulating form, cICAM-1, has been identified in serum.
- Uveitis encompasses diverse inflammatory eye conditions.
Purpose of the Study:
- To investigate circulating cICAM-1 serum levels in various uveitis subtypes.
- To compare cICAM-1 levels between uveitis patients and healthy controls.
Main Methods:
- Serum cICAM-1 levels were measured in 132 uveitis patients and controls.
- A monoclonal antibody-based ELISA was employed for quantification.
- Statistical analysis included multiple variance analysis and the Student Newmann-Keuls test.
Main Results:
- Significantly elevated serum cICAM-1 levels were observed in HLA-B27 negative anterior uveitis (n=31), intermediate uveitis (n=25), and sarcoidosis (n=18) patients.
- No significant difference in cICAM-1 levels was found in HLA-B27 positive acute anterior uveitis, Fuchs heterochromic cyclitis, or ocular toxoplasmosis patients compared to controls.
Conclusions:
- Elevated cICAM-1 may serve as a biomarker for specific inflammatory uveitis conditions.
- Further research can explore the diagnostic and prognostic value of cICAM-1 in ophthalmology.
Abstract:
Intercellular Adhesion Molecule-1 (ICAM-1) is a cytokine-inducible adhesion molecule expressed on cells of multiple lineages at sites of inflammation. Recently a truncated form of ICAM-1 has been discovered to be circulating in serum. This study reports on circulating serum (cICAM-1) levels in 132 uveitis patients (HLA-B 27 pos. acute anterior uveitis (AAU); HLA-B27 neg. anterior uveitis (AU); intermediate uveitis (IU); heterochromic cyclitis Fuchs (HCF); sarcoidosis; Toxoplasmosis). Measurement of circulating ICAM-1 serum levels was performed using a monoclonal antibody based ELISA, with healthy blood donors serving as the control group. Applying multiple variance analysis and the Student Newmann-Keuls test we found a statistically significant elevation of serum cICAM-1 level in the HLA-B 27 neg. AU group (n:31), in the IU group (n:25) and in patients with sarcoidosis (n:18). Serum levels of HLA-B27 pos. AAU patients, patients with HCF and patients suffering from ocular toxoplasmosis did not differ significantly from levels of the control group.
