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A monoclonal antibody that recognizes a formalin-resistant epitope on the p 24 core protein of HIV-1

G Kaluza1, W R Willems, J Lohmeyer

  • 1Institut für Med. Virologie, Justus-Liebig-Universität, Giessen, FRG.

Pathology, Research and Practice
|February 1, 1992
PubMed

Insights

A new mouse antibody, H-11, targets the HIV-1 p24 protein. It is effective for detecting human immunodeficiency virus type 1 in fixed tissue samples.

Area of Science:

  • Immunology
  • Virology
  • Pathology

Background:

  • Human immunodeficiency virus type 1 (HIV-1) infection requires sensitive detection methods.
  • The p24 core protein is a key viral antigen for HIV-1 identification.

Purpose of the Study:

  • To characterize a novel murine monoclonal antibody, H-11, targeting the HIV-1 p24 core protein.
  • To evaluate the utility of H-11 for detecting HIV-1 in various biological samples, particularly fixed tissues.

Main Methods:

  • Radioimmunoprecipitation
  • Immunofluorescence assays
  • Western blot analysis
  • Immunocytochemistry
  • Immunohistochemistry

Main Results:

  • The H-11 antibody specifically binds to the p24 core protein of HIV-1.
  • The antibody demonstrated high suitability for detecting HIV-1.
  • Successful application in formalin-fixed, paraffin-embedded tissues was confirmed.

Conclusions:

  • The H-11 monoclonal antibody is a valuable tool for HIV-1 research.
  • Its efficacy in immunohistochemistry makes it ideal for diagnosing HIV-1 in clinical pathology settings.

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