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[Binding of LPS to CD14]
U Grunwald1, G Oeser, H D Schröder
1Abteilung Medizinische Immunologie, Universität Greifswald.
Immunitat Und Infektion
|July 1, 1992
Insights
Cluster of differentiation 14 (CD14), a monocyte surface glycoprotein, acts as an endotoxin receptor. Its binding to lipopolysaccharide (LPS) is facilitated by LPS-binding protein (LBP) in serum.
Area of Science:
- Immunology
- Cell Biology
Background:
- CD14 (cluster of differentiation No. 14) is a 53 kDa glycoprotein found on monocyte surfaces.
- It was identified in 1990 as a key receptor for endotoxin.
Purpose of the Study:
- To elucidate the mechanism of endotoxin binding to CD14.
- To understand the role of serum proteins in this interaction.
Main Methods:
- Monoclonal antibody characterization of CD14.
- Functional assays investigating endotoxin-CD14 interaction.
Main Results:
- CD14 functions as an endotoxin receptor on monocytes.
- LPS-binding protein (LBP) in serum mediates endotoxin binding to CD14.
Conclusions:
- CD14 is crucial for monocyte recognition of endotoxins.
- LBP is essential for the CD14-mediated endotoxin binding pathway.
Abstract:
The 53 kDa glycoprotein CD14 (cluster of differentiation No. 14, defined by monoclonal antibodies of the 1st Leukocyte Typing Workshop) is expressed on monocyte surfaces and was identified 1990 as an endotoxin receptor (1). The binding of endotoxin to CD14 was mediated by a LPS-binding protein (LBP) present in serum.