Related Experiment Video

Updated: Aug 8, 2026

A Method For Production of Recombinant mCD1d Protein in Insect Cells.
09:41

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

Published on: December 11, 2007

Intracellular expression of CD1 molecules on PHA-activated normal T lymphocytes

M C Salamone1, L Fainboim

  • 1Laboratorio de Inmunogenética, Hospital de Clínicas José de San Martín, Buenos Aires, Argentina.

Immunology Letters
|June 1, 1992
PubMed

Insights

Researchers investigated CD1 antigen expression on activated human T cells. CD1 molecules were found intracellularly, not on the cell surface, peaking 72 hours after activation.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD1 molecules are important in T cell activation and antigen presentation.
  • Understanding CD1 expression dynamics is crucial for T cell immunology.

Purpose of the Study:

  • To analyze the intracellular and cell surface expression of CD1 antigens on PHA-activated human T cells.
  • To determine the kinetic expression profile of CD1 isotypes following T cell activation.

Main Methods:

  • Immunoperoxidase (IPx) and indirect immunofluorescence (IIF) techniques were employed.
  • Analysis was performed on PHA-activated human T cells from 13 healthy donors.

Main Results:

  • CD1 isotypes (CD1a, CD1b, CD1c) were detected intracellularly in activated T cells.
  • Cell surface expression of CD1 was not observed by IIF at 72 hours post-activation.
  • Intracellular CD1 expression commenced 48 hours after activation, with peak expression at 72 hours, contrasting with earlier CD25 detection.

Conclusions:

  • PHA-activated human T cells express CD1 molecules primarily in the cytoplasm, not on the cell surface.
  • The kinetics of CD1 expression differ from other activation markers like CD25.

More Related Videos

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells13:18

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

Published on: December 29, 2012

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

Related Concept Videos

Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Antigen Presenting Cells01:22

Antigen Presenting Cells

The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...